公开(公告)号：US20140173780A1

公开(公告)日：2014-06-19

申请号：US14133370

申请日：2013-12-18

Applicant: DOW AGROSCIENCES LLC

Inventor： Dayakar Pareddy ,  Sivarama R. Chennareddy ,  Tatyana Minnicks ,  Olga Karpova ,  David Griffin ,  Jayakumar P. Samuel ,  Kelley A. Smith ,  Rodrigo Sarria-Millan ,  Tejinder Kumar Mall

IPC: C12N15/82

CPC classification number: C12N15/8286 ,  A01H5/00 ,  C12N15/8205 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8283

Abstract: A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.