公开(公告)号：US20140173780A1

公开(公告)日：2014-06-19

申请号：US14133370

申请日：2013-12-18

Applicant: DOW AGROSCIENCES LLC

Inventor： Dayakar Pareddy ,  Sivarama R. Chennareddy ,  Tatyana Minnicks ,  Olga Karpova ,  David Griffin ,  Jayakumar P. Samuel ,  Kelley A. Smith ,  Rodrigo Sarria-Millan ,  Tejinder Kumar Mall

IPC: C12N15/82

CPC classification number: C12N15/8286 ,  A01H5/00 ,  C12N15/8205 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8283

Abstract: A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.

公开(公告)号：US20140173774A1

公开(公告)日：2014-06-19

申请号：US14134883

申请日：2013-12-19

Applicant: Dow AgroSciences LLC

Inventor： Dayakar Pareddy ,  Sivarama R. Chennareddy ,  Tatyana Minnicks ,  Olga Karpova ,  David Griffin ,  Jayakumar P. Samuel ,  Kelley Smith ,  Rodrigo Sarria-Milan

IPC: C12N15/82 ,  A01H5/00

CPC classification number: C12N15/8286 ,  A01H5/00 ,  C12N15/8205 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8283

Abstract: A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.

Abstract translation: 公开了一种用于土壤杆菌介导的大豆种系转化的方法，包括用含有转基因的根癌土壤杆菌感染具有一部分胚轴的分裂大豆种子。 所述方法还可以包括通过在选择培养基上体外包含部分胚胎轴的裂解大豆种子转化产生的外植体再生。

公开(公告)号：US20140283225A1

公开(公告)日：2014-09-18

申请号：US14211091

申请日：2014-03-14

Applicant: Dow Agrosciences LLC

Inventor： Jayakumar Pon Samuel ,  Sivarama Reddy Chennareddy ,  Andy Bowling ,  John Whitteck ,  Tatyana Minnicks ,  Dayakar Pareddy ,  Kerrm Y. Yau ,  Kelley A. Smith

IPC: G01N33/569

CPC classification number: C12N15/821

Abstract: The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants which are comprised of a combination of soybean non-germline transformants and soybean germline transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are detected and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the transformation process.

Abstract translation: 本公开部分地涉及从大豆非种系转化体和大豆种系转化体的组合组成的大豆转化体群体中鉴定大豆种系转化体的方法。 大豆非种系转化体在转化过程的早期被鉴定和消除。 检测并选择大豆种系转化体用于培养成熟大豆植物。 该方法易于在转化过程的早期筛选和获得大豆种系转化体。