公开(公告)号：US10947555B2

公开(公告)日：2021-03-16

申请号：US15288406

申请日：2016-10-07

Applicant: Dow AgroSciences LLC

Inventor： Terry Wright ,  Justin Lira ,  Donald Merlo ,  Nicole Arnold

IPC: C12N15/82 ,  C12N9/02

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

公开(公告)号：US11299745B1

公开(公告)日：2022-04-12

申请号：US17403476

申请日：2021-08-16

Applicant: DOW AGROSCIENCES LLC

Inventor： Terry Wright ,  Justin Lira ,  Donald Merlo ,  Nicole Arnold

IPC: C12N15/82 ,  C12N9/02

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

公开(公告)号：US10174337B2

公开(公告)日：2019-01-08

申请号：US14820893

申请日：2015-08-07

Applicant: Dow AgroSciences LLC

Inventor： Terry Wright ,  Justin Lira ,  Donald Merlo ,  Nicole Arnold

IPC: C12N15/82 ,  C12N9/02

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

公开(公告)号：US11098322B2

公开(公告)日：2021-08-24

申请号：US16279055

申请日：2019-02-19

Applicant: DOW AGROSCIENCES LLC

Inventor： Yunxing Cory Cui ,  Jill Bryan ,  Donald Maum ,  Greg Gilles ,  Terry Wright ,  Jennifer Hamilton ,  Nicole Arnold ,  Nathan VanOpdorp ,  Tina Kaiser ,  Ning Zhou

IPC: C12N15/82 ,  C12Q1/6895 ,  C12N9/02

Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (or corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.

公开(公告)号：US10323287B2

公开(公告)日：2019-06-18

申请号：US14989787

申请日：2016-01-06

Applicant: DOW AGROSCIENCES LLC

Inventor： Yunxing Cory Cui ,  Jill Bryan ,  Donald Maum ,  Greg Gilles ,  Terry Wright ,  Jennifer Hamilton ,  Nicole Arnold ,  Nathan VanOpdorp ,  Tina Kaiser ,  Ning Zhou

IPC: C12Q1/68 ,  C12N15/82 ,  C12Q1/6895 ,  A01H5/10 ,  C12N9/02

Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.

公开(公告)号：US09422569B2

公开(公告)日：2016-08-23

申请号：US13674606

申请日：2012-11-12

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Terry Wright ,  Manju Gupta

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8222 ,  C12N15/8286 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bidirectional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bidirectional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建物包含至少一个与多个基因表达盒的双向启动子连接。 在一些实施方案中，提供的构建体和方法使用基于来自玉米泛素-1基因的最小核心启动子元件或其功能等同物的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US11149283B2

公开(公告)日：2021-10-19

申请号：US17175966

申请日：2021-02-15

Applicant: DOW AGROSCIENCES LLC

Inventor： Terry Wright ,  Justin Lira ,  Donald Merlo ,  Nicole Arnold

IPC: C12N15/82 ,  C12N9/02

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

公开(公告)号：US20130254943A1

公开(公告)日：2013-09-26

申请号：US13674606

申请日：2012-11-12

Applicant: DOW AGROSCIENCES LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Terry Wright ,  Manju Gupta

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8222 ,  C12N15/8286 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 提供的构建物包含至少一个双重启动子连接到多个基因表达盒。 在一些实施方案中，提供的构建体和方法使用基于来自玉米泛素-1基因的最小核心启动子元件或其功能等同物的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。