公开(公告)号：US11236350B2

公开(公告)日：2022-02-01

申请号：US16139112

申请日：2018-09-24

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Sara Bennett ,  Andrew J. Bowling ,  Alicia Walker ,  Cory M. Larsen ,  Heather Pence ,  Staci Weaver ,  Ronnie Hampton, Jr.

IPC: C12N15/82 ,  A01H6/60

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Glycine max egg cell gene. Some embodiments relate to a promoter or a 5′ UTR from a Glycine max egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR or a terminator from a Glycine max egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US10968455B2

公开(公告)日：2021-04-06

申请号：US16271986

申请日：2019-02-11

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Wei Chen

IPC: C12N15/82 ,  C12N15/00

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G144030 promoter. Some embodiments relate to a Zea mays GRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G144030 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US10457955B2

公开(公告)日：2019-10-29

申请号：US15698708

申请日：2017-09-08

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Pierluigi Barone ,  Daren Hemingway ,  Emily Etchison ,  Andrew Asberry ,  Heather Pence ,  Andrew J. Bowling

IPC: C12N15/82 ,  C12Q1/6895

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum (Pavir.Cb02009) egg cell gene. Some embodiments relate to a promoter from a Panicum virgatum (Pavir.Cb02009) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR from a Panicum virgatum (Pavir.Cb02009) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US20190161761A1

公开(公告)日：2019-05-30

申请号：US16271986

申请日：2019-02-11

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Wei Chen

IPC: C12N15/82

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G144030 promoter. Some embodiments relate to a Zea mays GRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G144030 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US20170334956A1

公开(公告)日：2017-11-23

申请号：US15484581

申请日：2017-04-11

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Wei Chen

IPC: C07K14/415 ,  C12N15/82 ,  C12N15/00

CPC classification number: C12N15/8216 ,  C12N15/8222 ,  C12N15/8225 ,  C12N15/8226 ,  C12N15/8227 ,  C12N15/823

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G144030 promoter. Some embodiments relate to a Zea mays GRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G144030 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US09725730B2

公开(公告)日：2017-08-08

申请号：US14587762

申请日：2014-12-31

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC: C12N15/82 ,  C12N15/10

CPC classification number: C12N15/8216 ,  C12N15/10

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v2.

公开(公告)号：US09708618B2

公开(公告)日：2017-07-18

申请号：US14472965

申请日：2014-08-29

Applicant: Dow AgroSciences, LLC

Inventor： Sandeep Kumar ,  Jeffrey Beringer

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8216 ,  C07K14/415

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3′-UTRs, isolated from Brachypodium distachyon ubiquitin genes.

公开(公告)号：US20150203856A1

公开(公告)日：2015-07-23

申请号：US14416811

申请日：2013-08-08

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Diaa Alabed

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8218

Abstract: Provided are methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 3′ untranslated regions of genes encoding ubiquitin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional or gene targeting procedures, resulting in enhanced expression of the selected coding region. In some embodiments, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

Abstract translation: 提供了用于增强转基因植物和植物组织中重组核酸序列表达的方法，载体和基因构建体。 根据本发明，获得核酸序列和/或衍生自编码泛素蛋白的基因的3'非翻译区并且被工程化成位于载体的选定编码区的相应部分。 载体构建体可以通过常规或基因靶向程序引入植物和/或植物组织，导致所选编码区的表达增强。 在一些实施方案中，所选择的编码区是表达一种或多种已知赋予转基因植物和/或植物组织杀虫活性水平的蛋白质的嵌合基因或基因片段。

公开(公告)号：US20130254943A1

公开(公告)日：2013-09-26

申请号：US13674606

申请日：2012-11-12

Applicant: DOW AGROSCIENCES LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Terry Wright ,  Manju Gupta

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8222 ,  C12N15/8286 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 提供的构建物包含至少一个双重启动子连接到多个基因表达盒。 在一些实施方案中，提供的构建体和方法使用基于来自玉米泛素-1基因的最小核心启动子元件或其功能等同物的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US20210171968A1

公开(公告)日：2021-06-10

申请号：US17166285

申请日：2021-02-03

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Marcelo Ariel German ,  Tristan E Coram ,  Terry R. Wright

IPC: C12N15/82

Abstract: This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in male plant tissues, for example, to provide an economical male sterility system of hybrid seed production.