公开(公告)号：US20140248397A1

公开(公告)日：2014-09-04

申请号：US13842344

申请日：2013-03-15

Applicant: DSM IP ASSETS B.V.

Inventor： Rene Marcel DE JONG ,  Hanna Elisabet ABBAS

IPC: A21D8/04 ,  C12N9/28

CPC classification number: A21D8/042 ,  C12N9/2417 ,  C12Y302/01001

Abstract: A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 4, 6, 13, 14, 15, 16, 20, 45, 47, 51, 54, 61, 68, 69, 70, 71, 72, 73, 74, 75, 77, 78, 80, 82, 87, 88, 94, 95, 100, 103, 104, 117, 124, 125, 126, 128, 130, 133, 134, 136, 143, 144, 146, 168, 174, 177, 178, 183, 186, 188, 189, 190, 194, 195, 199, 200, 201, 204, 207, 210, 214, 217, 219, 222, 225, 227, 233, 234, 235, 236, 240, 251, 252, 254, 258, 259, 260, 261, 262, 263, 264, 266, 267, 269, 271, 273, 281, 282, 283, 284, 286, 288, 299, 322, 323, 325, 327, 328, 331, 334, 350, 356, 358, 367, 370, 371, 374, 377, 378, 388, 391, 414, 421, 422, 445, 450, 467, 488, 505, 536, 548, 583, 588, 603, 637, 648, 651, 652, 660, 676, 677,said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having alpha-amylase activity. Such a variant polypeptide may be used in the preparation of a baked product.

Abstract translation: 具有α-淀粉酶活性的变体多肽，其中所述变体具有当与包含SEQ ID NO：2所示的序列的α-淀粉酶比对时的氨基酸序列包含至少一个对应于 任何氨基酸4,6,13,14,15,16,20,45,47,51,54,61,68,69,70,71,72,73,74,75,77,78,80， 82,87,88,94,95,100,103,104,117,124,125,126,128,130,133,134,136,143,144,146,168,174,177,178,183， 186,188,189,190,194,195,199,200,2201,204,207,210,214,217,219,222,225,227,233,234,235,236,240,251,252,252,218,218,218,218,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,2 254,258,258,260,261,262,263,264,266,262,263,264,266,276,263,264,266,276,263,267,271,273,281,282,283,284,286,288,29,322,236,265,276,262,263,264,266,262,263,264,266,266,263,264,266,266,263,264,266,266,263,264,266,266,263,264,266,266,263,264,266,266,263 331,334,350,356,358,367,370,371,374,377,378,388,391,414,421,422,445,450,467,488,505,536,548,583,558,538,348,336,350,356,358,367,350,356,358,367,370,356,358,367,370,356,358,367,375,356,358,367,370,356,358,367,370,356,358,367,375,356,358,367,375,356,358 603,637,648,651,652,660,676,677，所述位置参考SEQ ID NO：2定义，其中变体ha 与具有α-淀粉酶活性的参考多肽相比，具有一个或多个改变的特性。 这样的变体多肽可用于制备焙烤产品。

公开(公告)号：US20170260514A1

公开(公告)日：2017-09-14

申请号：US15501149

申请日：2015-08-06

Applicant: DSM IP Assets B.V.

Inventor： Hanna Elisabet ABBAS ,  Jan Metske VAN DER LAAN ,  Johannes Gustaaf Ernst VAN LEEUWEN

IPC: C12N9/30 ,  A21D2/26

CPC classification number: C12N9/242 ,  A21D2/26 ,  C12Y302/01001

Abstract: The present invention relates to an alpha amylase enzyme and to its use in the food, feed and non-food industry.

公开(公告)号：US20150376588A1

公开(公告)日：2015-12-31

申请号：US14767406

申请日：2014-02-27

Applicant: DSM IP ASSETS B.V.

Inventor： Rene Marcel DE JONG ,  Hanna Elisabet ABBAS ,  Evert Tjeerd VAN RIJ

IPC: C12N9/28 ,  A21D8/04

CPC classification number: A21D8/042 ,  C12N9/2417 ,  C12Y302/01001

Abstract: A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue. Such a variant polypeptide may be used in the preparation of a baked product.

Abstract translation: 具有α-淀粉酶活性的变体多肽，其中所述变体具有当与包含SEQ ID NO：2所示的序列的α-淀粉酶比对时的氨基酸序列包含氨基酸残基的至少一个取代。 这样的变体多肽可用于制备焙烤产品。