公开(公告)号：US20170175130A1

公开(公告)日：2017-06-22

申请号：US15116933

申请日：2015-02-06

Applicant: DSM IP Assets B.V.

Inventor： Evert Tjeerd VAN RIJ ,  Martine SPAANS ,  Hendrikus Bernardus Carolus HILLEBRAND

IPC: C12N15/75 ,  C12N9/26 ,  C12N9/24 ,  C12N9/54 ,  C12P21/00

CPC classification number: C12N15/75 ,  C12N9/2411 ,  C12N9/2482 ,  C12N9/54 ,  C12P21/00 ,  C12P21/02 ,  C12Y302/01008 ,  C12Y302/01133 ,  C12Y304/21062 ,  C12Y304/24028

Abstract: The invention provides Bacillus host cell deficient in the production of a neutral protease and of a alkaline protease which host cell comprises a nucleic acid construct, wherein the nucleic acid construct comprises a polynucleotide encoding a compound of interest or encoding a compound involved in the synthesis of a compound of interest operably linked to a promoter sequence allowing for expression of the polynucleotide in the host cell, wherein the promoter comprises a bacteriophage promoter sequence, more preferably a bacteriophage SPO1 promoter sequence. Said host cell can advantageously be used in a method of production of a compound of interest.

公开(公告)号：US20190185830A1

公开(公告)日：2019-06-20

申请号：US16305348

申请日：2017-06-08

Applicant: DSM IP Assets B.V.

Inventor： Rene Marcel DE JONG ,  Willem BIJLEVELD ,  Evert Tjeerd VAN RIJ

IPC: C12N9/20 ,  A23C13/16 ,  A23C15/12 ,  A23C19/06 ,  A23C19/082

CPC classification number: C12N9/20 ,  A23C13/16 ,  A23C15/123 ,  A23C19/063 ,  A23C19/082 ,  C12Y301/01003

Abstract: The present invention relates to a polypeptide having lipase activity wherein the polypeptide, which, when aligned with a polypeptide according to SEQ ID NO: 1, comprises at least one amino acid substitution resulting in Ser (S), Ala (A) or Leu (L) at position 246, Trp (W) at position 307, Leu (L) at position 345, Ile (I) at position 365, and/or Phe (F) at position 534, wherein the position is defined with reference to SEQ ID NO: 1, wherein Ala(A) at position 1 in SEQ ID NO: 1 is counted as number 1 and a method for preparing the polypeptide.The present invention further relates to a process for preparing a food product wherein a polypeptide according to the present invention is used.

公开(公告)号：US20250152645A1

公开(公告)日：2025-05-15

申请号：US18838938

申请日：2023-02-17

Applicant: DSM IP ASSETS B.V.

Inventor： Carol ROA-ENGEL ,  Mickel Leonardus August JANSEN ,  Evert Tjeerd VAN RIJ ,  Hendrik Jan NOORMAN ,  Ester SANTIGOSA CULI

IPC: A61K36/064 ,  A23K10/16 ,  A23K20/147 ,  A23K50/80 ,  C12N1/16 ,  C12R1/645

Abstract: The present invention relates to an animal feed comprising up to 20% (w/w) yeast single cell protein product, wherein the yeast single cell protein product comprises Saccharomycesales yeast cells, as well as to the use of said animal feed for feeding an animal and/or for increasing body weight of an animal.

公开(公告)号：US20230374443A1

公开(公告)日：2023-11-23

申请号：US18030026

申请日：2021-10-05

Applicant: DSM IP ASSETS B.V.

Inventor： Hans Marinus Charles Johannes DE BRUIJN ,  Phillip E. BUREMAN ,  Evert Tjeerd VAN RIJ ,  René Marcel DE JONG

IPC: C12N1/18 ,  C12P7/06 ,  C12N9/06

CPC classification number: C12N1/185 ,  C12P7/06 ,  C12N9/0044 ,  C12R2001/865

Abstract: A Saccharomyces yeast cell, preferably a Saccharomyces cerevisae yeast cell, comprising one or more nucleic acid sequences encoding an enzyme having NADH-dependent nitrate reductase activity and/or one or more nucleic acid sequences encoding an enzyme having NADH-dependent nitrite reductase activity. An anaerobic fermentation process for the production of a fermentation product using such Saccharomyces yeast cell and a fermentation co-product produced.

公开(公告)号：US20180134986A1

公开(公告)日：2018-05-17

申请号：US15564275

申请日：2016-04-08

Applicant: DSM IP ASSETS B.V.

Inventor： Arjen SEIN ,  Rene Marcel DE JONG ,  Evert Tjeerd VAN RIJ ,  Willem BIJLEVELD ,  Maarten Hotse WILBRINK

IPC: C11B3/00 ,  C12N9/16 ,  C12P7/64

CPC classification number: C11B3/003 ,  C12N9/16 ,  C12P7/20 ,  C12P7/64 ,  C12P7/6445 ,  C12Y301/04003

Abstract: The present invention relates to a polypeptide having phospholipase C activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1. A process for degumming a vegetable oil comprising contacting a vegetable oil comprising phospholipids with a polypeptide having phospholipase C activity of the invention or a composition of the invention, wherein phospholipids are hydrolysed into diacylglycerol and phosphate ester and/or phosphate, separating the phosphate ester and/or phosphate from the vegetable oil wherein a degummed vegetable oil is obtained.

公开(公告)号：US20180007917A1

公开(公告)日：2018-01-11

申请号：US15716218

申请日：2017-09-26

Applicant: DSM IP ASSETS B.V.

Inventor： Rene Marcel DE JONG ,  Hanna Elsabet ABBAS ,  Evert Tjeerd VAN RIJ

IPC: A21D8/04 ,  C12N9/28

CPC classification number: A21D8/042 ,  A21D10/005 ,  C12N9/2417 ,  C12Y302/01001

Abstract: A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue. Such a variant polypeptide may be used in the preparation of a baked product.

公开(公告)号：US20150376588A1

公开(公告)日：2015-12-31

申请号：US14767406

申请日：2014-02-27

Applicant: DSM IP ASSETS B.V.

Inventor： Rene Marcel DE JONG ,  Hanna Elisabet ABBAS ,  Evert Tjeerd VAN RIJ

IPC: C12N9/28 ,  A21D8/04

CPC classification number: A21D8/042 ,  C12N9/2417 ,  C12Y302/01001

Abstract: A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue. Such a variant polypeptide may be used in the preparation of a baked product.

Abstract translation: 具有α-淀粉酶活性的变体多肽，其中所述变体具有当与包含SEQ ID NO：2所示的序列的α-淀粉酶比对时的氨基酸序列包含氨基酸残基的至少一个取代。 这样的变体多肽可用于制备焙烤产品。