公开(公告)号：US20210095325A1

公开(公告)日：2021-04-01

申请号：US17050639

申请日：2019-05-21

Applicant: DSM IP ASSETS B.V.

Inventor： Otto Martin LEHMANN ,  Harald PICHLER ,  Holly STOLTERFOHT

IPC: C12P33/00 ,  C12N9/10 ,  C12N9/02

Abstract: The present invention is related to modified sterol acyltransferase enzymes with improved activity and/or specificity towards acylation of the vitamin D3 precursor 7-dehydrocholesterol (7-DHC) to be used in biotechnological production of vitamin D3. The invention further relates to a host strain expressing said modified enzymes and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.