公开(公告)号：US20220204947A1

公开(公告)日：2022-06-30

申请号：US17204018

申请日：2021-03-17

Applicant: DSM IP ASSETS B.V.

Inventor： Paulus Petrus DE WAAL ,  Ingrid Maria VUGT - VAN LUTZ ,  Jozef Petrus Johannes SCHMITZ ,  Hans Marinus Charles Johannes DE BRUIJN

IPC: C12N9/04 ,  C12N9/12 ,  C12N9/88 ,  C12P7/14

Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.

公开(公告)号：US20220325253A9

公开(公告)日：2022-10-13

申请号：US17204018

申请日：2021-03-17

Applicant: DSM IP ASSETS B.V.

Inventor： Paulus Petrus DE WAAL ,  Ingrid Maria VUGT - VAN LUTZ ,  Jozef Petrus Johannes SCHMITZ ,  Hans Marinus Charles Johannes DE BRUIJN

IPC: C12N9/04 ,  C12N9/12 ,  C12N9/88 ,  C12P7/14

Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.

公开(公告)号：US20200239914A1

公开(公告)日：2020-07-30

申请号：US16651212

申请日：2018-09-25

Applicant: DSM IP ASSETS B.V.

Inventor： Paulus Petrus DE WAAL ,  Ingrid Maria VUGT - VAN LUTZ

IPC: C12P7/10 ,  C12N9/34 ,  C12N9/88 ,  C12N9/04

Abstract: The invention relates to a recombinant yeast comprising a recombinant yeast comprising a nucleotide sequence coding for a glycerol dehydrogenase, a nucleotide sequence coding for a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39); a nucleotide sequence coding for a phosphoribulokinasey (EC 2.7.1.19); a nucleotide sequence allowing the expression of a glucoamylase (EC 3.2.1.20 or 3.2.1.3); and optionally a nucleotide sequence coding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.

公开(公告)号：US20210317420A1

公开(公告)日：2021-10-14

申请号：US17345610

申请日：2021-06-11

Applicant: DSM IP ASSETS B.V.

Inventor： Paulus Petrus DE WAAL ,  Ingrid Maria VUGT - VAN LUTZ ,  Jozef Petrus Johannes SCHMITZ ,  Hans Marinus Charles Johannes DE BRUIJN

IPC: C12N9/04 ,  C12N9/88 ,  C12N9/12 ,  C12P7/14

Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter, wherein the recombinant yeast comprises overexpression of one or more PPP-genes. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.

公开(公告)号：US20200283805A1

公开(公告)日：2020-09-10

申请号：US16649284

申请日：2018-09-25

Applicant: DSM IP ASSETS B.V.

Inventor： Paulus Petrus DE WAAL ,  Ingrid Maria VUGT - VAN LUTZ ,  Jozef Petrus Johannes SCHMITZ ,  Hans Marinus Charles Johannes DE BRUIJN

IPC: C12P7/10 ,  C12N9/10 ,  C12N9/02 ,  C12N9/04 ,  C07K14/395

Abstract: The invention relates to a process for the production of ethanol from a composition comprising at least glucose comprising fermenting said composition in the presence of a recombinant yeast; and recovering the ethanol, wherein said yeast comprises one or more genes coding for an enzyme having glycerol dehydrogenase activity, one or more genes coding for an enzyme having dihydroxyacetone kinase activity (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); one or more genes coding for an enzyme in an acetyl-CoA-production pathway and one or more genes coding for an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2), and optionally one or more genes coding for a glycerol transporter, wherein the composition comprises an amount of undissociated acetic acid of 10 mM or less. A recombinant yeast having the genes as described above is particularly sensitive towards acetic acid, and the ethanol yield rapidly decreases when the composition contains more than 10 mM undissociated acetic acid.

公开(公告)号：US20190309268A1

公开(公告)日：2019-10-10

申请号：US16470387

申请日：2017-12-18

Applicant: DSM IP ASSETS B.V.

Inventor： Paulus Petrus DE WAAL ,  Ingrid Maria VUGT - VAN LUTZ ,  Jozef Petrus Johannes SCHMITZ ,  Hans Marinus Charles Johannes DE BRUIJN

IPC: C12N9/04 ,  C12P7/06 ,  C12N9/12 ,  C12N9/88 ,  C12N9/16

Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.