公开(公告)号：US11326172B2

公开(公告)日：2022-05-10

申请号：US16763394

申请日：2018-11-12

Applicant: DSM IP ASSETS B.V.

Inventor： Antonius Jeroen Adriaan Van Maris ,  Jacobus Thomas Pronk ,  Maarten Dirk Verhoeven

IPC: C12N15/00 ,  C12N15/70 ,  C12N9/90 ,  C12P7/06 ,  C12N15/52 ,  C07K14/435 ,  C12N9/12 ,  C12P7/14

Abstract: The invention relates to a variant of a parent polypeptide which is preferably a hexose transporter, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of SEQ ID NO: 1, comprises a substitution of amino acids N376 and T89, the positions of said amino acids being defined with reference to the amino acid sequence of SEQ ID NO: 1. Said polypeptide is suitable in the production of ethanol from arabinose-rich biomass such as corn stover and corn starch.

公开(公告)号：US10689670B2

公开(公告)日：2020-06-23

申请号：US16309422

申请日：2017-06-13

Applicant: DSM IP ASSETS B.V.

Inventor： Ioannis Papapetridis ,  Antonius Jeroen Adriaan Van Maris ,  Jacobus Thomas Pronk ,  Paulus Petrus De Waal ,  Niels Lauret ,  Maarten Dirk Verhoeven ,  Jozef Petrus Johannes Schmitz

IPC: C12P7/06 ,  C12N15/81 ,  C12N1/16 ,  C12R1/645 ,  C12N9/12 ,  C12N9/88

Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.

公开(公告)号：US11198847B2

公开(公告)日：2021-12-14

申请号：US16966741

申请日：2019-01-31

Applicant: DSM IP ASSETS B.V.

Inventor： Antonius Jeroen Adriaan Van Maris ,  Jacobus Thomas Pronk ,  Maarten Dirk Verhoeven ,  Ioannis Papapetridis

IPC: C12N15/52 ,  C12N1/18 ,  C12N1/22 ,  C12N9/04 ,  C12N9/12 ,  C12N9/90 ,  C12N9/92 ,  C12P1/02

Abstract: The invention relates to a method for preparing a yeast which is capable of simultaneously fermenting a pentose and a hexose sugar, the method comprising providing a yeast which comprises: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway, a disruption of a gene encoding a ribulose-phosphate 3-epimerase and a disruption of a gene encoding a glucose-6-phosphate isomerase, and one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and subjecting the yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate when grown on a media comprising a hexose and at least one pentose sugar, so as to obtain an evolved yeast.

公开(公告)号：US11186850B2

公开(公告)日：2021-11-30

申请号：US16858163

申请日：2020-04-24

Applicant: DSM IP ASSETS B.V.

Inventor： Ioannis Papapetridis ,  Antonius Jeroen Adriaan Van Maris ,  Jacobus Thomas Pronk ,  Paulus Petrus De Waal ,  Niels Lauret ,  Maarten Dirk Verhoeven ,  Jozef Petrus Johannes Schmitz

IPC: C12P7/06 ,  C12N9/12 ,  C12N9/88 ,  C12N15/81 ,  C12N1/14 ,  C12N1/16 ,  C12R1/645

Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.