公开(公告)号：US20190249201A1

公开(公告)日：2019-08-15

申请号：US16309422

申请日：2017-06-13

Applicant: DSM IP ASSETS B.V.

Inventor： Ioannis PAPAPETRIDIS ,  Antonius Jeroen Adriaan VAN MARIS ,  Jacobus Thomas PRONK ,  Paulus Petrus DE WAAL ,  Niels LAURET ,  Maarten Dirk VERHOEVEN ,  Jozef Petrus Johannes SCHMITZ

IPC: C12P7/06 ,  C12R1/645 ,  C12N1/16 ,  C12N9/88 ,  C12N9/12

CPC classification number: C12P7/06 ,  C12N1/16 ,  C12N9/1205 ,  C12N9/88 ,  C12N15/81 ,  C12R1/645 ,  C12Y207/01019 ,  C12Y401/01039 ,  Y02E50/17

Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.

公开(公告)号：US20200255871A1

公开(公告)日：2020-08-13

申请号：US16858163

申请日：2020-04-24

Applicant: DSM IP ASSETS B.V.

Inventor： Ioannis PAPAPETRIDIS ,  Antonius Jeroen Adriaan VAN MARIS ,  Jacobus Thomas PRONK ,  Paulus Petrus DE WAAL ,  Niels LAURET ,  Maarten Dirk VERHOEVEN ,  Jozef Petrus Johannes SCHMITZ

IPC: C12P7/06 ,  C12N15/81 ,  C12R1/645 ,  C12N1/16 ,  C12N9/88 ,  C12N9/12

Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.