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公开(公告)号：US20210230614A1
公开(公告)日：2021-07-29
申请号：US17176073
申请日：2021-02-15
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas PRONK , Antonius Jeroen Adriaan VAN MARIS , Victor Gabriel GUADALUPE MEDINA
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US20190249201A1
公开(公告)日：2019-08-15
申请号：US16309422
申请日：2017-06-13
Applicant: DSM IP ASSETS B.V.
Inventor： Ioannis PAPAPETRIDIS , Antonius Jeroen Adriaan VAN MARIS , Jacobus Thomas PRONK , Paulus Petrus DE WAAL , Niels LAURET , Maarten Dirk VERHOEVEN , Jozef Petrus Johannes SCHMITZ
CPC classification number: C12P7/06 , C12N1/16 , C12N9/1205 , C12N9/88 , C12N15/81 , C12R1/645 , C12Y207/01019 , C12Y401/01039 , Y02E50/17
Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.
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公开(公告)号：US20220135989A1
公开(公告)日：2022-05-05
申请号：US17531218
申请日：2021-11-19
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas PRONK , Antonius Jeroen Adriaan VAN MARIS , Victor Gabriel GUADALUPE MEDINA
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US20210230613A1
公开(公告)日：2021-07-29
申请号：US17176040
申请日：2021-02-15
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas PRONK , Antonius Jeroen Adriaan VAN MARIS , Victor Gabriel GUADALUPE MEDINA
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US20200248194A1
公开(公告)日：2020-08-06
申请号：US16833342
申请日：2020-03-27
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas PRONK , Antonius Jeroen Adriaan VAN MARIS , Victor Gabriel GUADALUPE MEDINA
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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Patent Agency Ranking
公开(公告)号：US20210147859A1
公开(公告)日：2021-05-20
申请号：US17108027
申请日：2020-12-01
Applicant: DSM IP ASSETS B.V.
Inventor： Antonius Jeroen Adriaan VAN MARIS , Jacobus Thomas PRONK , Victor Gabriel GUADALUPE MEDINA , Hendrik Wouter WISSELINK
Abstract: The invention relates to a recombinant yeast cell, in particular a transgenic yeast cell, functionally expressing one or more recombinant, in particular heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK). The invention further relates to the use of carbon dioxide as an electron acceptor in a recombinant chemotrophic micro-organism, in particular a eukaryotic micro-organism.
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公开(公告)号：US20200299704A1
公开(公告)日：2020-09-24
申请号：US16893065
申请日：2020-06-04
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas PRONK , Antonius Jeroen Adriaan VAN MARIS , Victor Gabriel GUADALUPE MEDINA
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US20180291404A1
公开(公告)日：2018-10-11
申请号：US15766184
申请日：2016-10-03
Applicant: DSM IP Assets B.V.
Inventor： Ioannis PAPAPETRIDIS , Jacobus THomas PRONK , Antonius Jeroen Adriaan VAN MARIS , Paul KLAASSEN
Abstract: The present invention relates to a eukaryotic cell that is genetically modified comprising one or more heterologous gene encoding: a) D-glucose-6-phosphate dehydrogenase and/or b) 6-phosphogluconate dehydrogenase; and/or c) glucose dehydrogenase, gluconolactonase and gluconate kinase, wherein a), b) and glucose dehydrogenase in c) are NAD+ dependent.
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公开(公告)号：US20210002651A1
公开(公告)日：2021-01-07
申请号：US16763394
申请日：2018-11-12
Applicant: DSM IP ASSETS B.V.
IPC: C12N15/52 , C12N9/90 , C12N9/12 , C12P7/14 , C07K14/435
Abstract: The invention relates to a variant of a parent polypeptide which is preferably a hexose transporter, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of SEQ ID NO: 1, comprises a substitution of amino acids N376 and T89, the positions of said amino acids being defined with reference to the amino acid sequence of SEQ ID NO: 1. Said polypeptide is suitable in the production of ethanol from arabinose-rich biomass such as corn stover and corn starch.
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公开(公告)号：US20200377846A1
公开(公告)日：2020-12-03
申请号：US16966741
申请日：2019-01-31
Applicant: DSM IP ASSETS B.V.
Inventor： Antonius Jeroen Adriaan VAN MARIS , Jacobus Thomas PRONK , Maarten Dirk VERHOEVEN , Ioannis PAPAPETRIDIS
Abstract: A method for preparing a yeast capable of simultaneously fermenting a pentose and a hexose sugar, comprising: (a) providing a yeast comprising: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway; disruptions of a gene encoding a ribulose-phosphate 3-epimerase and of a gene encoding a glucose-6-phosphate isomerase; one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and a disruption of one or more genes encoding an NADPH dependent 6-phosphogluconate dehydrogenase, (b) subjecting said yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate obtain an evolved yeast; (d) restoring, in the evolved yeast, one or more of the disrupted genes, or: (d′) identifying genetic permutations in at least part of the genome of the evolved yeast by genome sequencing; (e) constructing an improved pentose and hexose-fermenting yeast comprising one or more said genetic permutations. Also described is a recombinant yeast comprising one or more heterologous genes of a pentose metabolic pathway, and a gene encoding a variant of a parent polypeptide, the variant comprising an amino acid sequence comprising at least one mutation, when aligned with the amino acid sequence in SEQ ID NO: 6.
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