公开(公告)号：US20170044556A1

公开(公告)日：2017-02-16

申请号：US15306120

申请日：2015-04-28

Applicant: DSM IP ASSETS B.V.

Inventor： Anita EMMERSTORFER ,  Tamara WRIESSNEGGER ,  Miriam WIMMER-TEUBENBACHER ,  Harald PICHLER ,  Monika MUELLER ,  Iwona KALUZNA ,  Martin SCHURMANN

IPC: C12N15/81 ,  C12N9/02 ,  C07K14/395 ,  C12P7/26

CPC classification number: C12N15/81 ,  C07K14/395 ,  C12N9/0042 ,  C12N9/0071 ,  C12N9/0073 ,  C12P7/02 ,  C12P7/26 ,  C12P19/56 ,  C12P33/00 ,  C12Y106/02004 ,  C12Y114/13121

Abstract: The present invention relates to a recombinant microorganism which is capable of expressing a cytochrome P450 reductase (CPR) and, optionally, a cytochrome P450 enzyme (CYP) and which is capable of overexpressing a Ice2p comprising the amino acid sequence set out in SEQ ID NO: 2 or a sequence having at least 50% sequence identity thereto. The invention further relates to use of Ice2p to stabilize expression of a cytochrome P450 reductase in a recombinant microorganism.

Abstract translation: 本发明涉及能够表达细胞色素P450还原酶（CPR）和任选的细胞色素P450酶（CYP）的重组微生物，其能够过表达包含SEQ ID NO中所示的氨基酸序列的Ice2p ：2或与其具有至少50％序列同一性的序列。 本发明还涉及使用Ice2p来稳定重组微生物中细胞色素P450还原酶的表达。