公开(公告)号：US12286658B2

公开(公告)日：2025-04-29

申请号：US18561258

申请日：2022-05-17

Applicant: DSM IP Assets B.V.

Inventor： Manos Papadakis

IPC: C12P19/18 ,  C12N9/10 ,  C12N15/52 ,  C12P19/00

Abstract: This invention relates to a method of producing one or more human milk oligosaccharides (HMOs), in particular LNT and/or LNnT, in a genetically engineered cell comprising an enhanced oligosaccharide transport capability. The genetically modified cell comprises a series of genetic modification which enable the production of one or more HMO(s), and a series of genetic modification that enhances the transport of lactose and produced HMO(s).

公开(公告)号：US12188056B2

公开(公告)日：2025-01-07

申请号：US18177070

申请日：2023-03-01

Applicant: DSM IP Assets B.V.

Inventor： Manos Papadakis

IPC: C12N9/10 ,  C07K14/24 ,  C12P19/04 ,  C12P19/18

Abstract: The present disclosure relates to the production of sialylated Human Milk Oligosaccharides (HMOs), in particular to the biosynthetic production of 3′-sialyllactose (3′SL), and to genetically engineered cells and methods suitable for said production.