公开(公告)号：US20130295607A1

公开(公告)日：2013-11-07

申请号：US13923481

申请日：2013-06-21

Applicant: DSM IP Assets B.V.

Inventor： Paul G. ROESSLER ,  T. Dave Matthews ,  Tom M. Ramseier ,  James G. Metz

IPC: C12N15/82

CPC classification number: C12N15/82 ,  C12N9/88 ,  C12N15/79 ,  C12P21/02 ,  C12Y202/01006

Abstract: Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.

Abstract translation: 公开了乙酰乳酸合酶，乙酰乳酸合成酶调节区，α-微管蛋白启动子，破囊壶菌聚酮合成酶（PKS）系统的启动子和来自破囊壶菌菌体的脂肪酸去饱和酶启动子的核酸和氨基酸序列。 还公开了可用于转录破囊索杆菌微生物的重组载体，以及转变破囊壶菌微生物的方法。 本发明的重组核酸分子可用于在破囊壶菌属微生物中表达外源核酸，以及用于在裂囊酵素微生物中的基因的缺失，突变或失活。

公开(公告)号：US20220170037A1

公开(公告)日：2022-06-02

申请号：US17669945

申请日：2022-02-11

Applicant: DSM IP Assets B.V. ,  DOW AgroSciences LLC

Inventor： Terence A. WALSH ,  Daniel GACHOTTE ,  Ann Owens MERLO ,  Paul G. ROESSLER ,  James George METZ ,  Scott BEVAN ,  Jerry M. KUNER

IPC: C12N15/82 ,  C12P7/6427

Abstract: The invention provides recombinant host organisms (e.g., plants) genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins (e.g., PPTase and/or ACoAS) that allow for and/or improve the production of PUFAs in the host organism. The present invention also relates to methods of making and using such organisms (e.g., to obtain PUFAs) as well as products obtained from such organisms (e.g., oil and/or seed).