公开(公告)号：US20240309316A1

公开(公告)日：2024-09-19

申请号：US18672979

申请日：2024-05-23

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Amy S. Johnson ,  Meghan E. Casey ,  Shadia Oshodi ,  Shawn Lawrence

IPC分类号： C12N5/00 ,  C12N15/79

CPC分类号： C12N5/0018 ,  C12N15/79 ,  C12N2500/33 ,  C12N2500/74 ,  C12N2500/80

摘要： The specification describes a composition comprising an improved eukaryotic cell culture medium, which can be used for the production of a protein of interest. TaXULne can be added to the serum-free media or chemically-defined media to increase the production of a protein of interest. Methods for recombinantly expressing high levels of protein using the media compositions are included.

公开(公告)号：US12070476B2

公开(公告)日：2024-08-27

申请号：US17584508

申请日：2022-01-26

申请人： Ramot at Tel-Aviv University Ltd. ,  The University Court of the University of Glasgow

发明人： Oded Rechavi ,  Shahar Bracha ,  Lilach Sheiner

IPC分类号： A61K35/68 ,  C07K14/45 ,  C12N9/00 ,  C12N15/65 ,  C12N15/79

CPC分类号： A61K35/68 ,  C07K14/45 ,  C12N9/93 ,  C12N15/65 ,  C12N15/79 ,  C07K2319/10 ,  C12Y603/05005

摘要： Provided are nucleic acid constructs, Toxoplasma comprising same, pharmaceutical compositions comprising same and methods using same for delivering a protein-of-interest to a tissue-of-interest of a subject, such as the CNS and further treating a pathology which is treatable by administration of a therapeutic polypeptide in a central nervous system of the subject.

公开(公告)号：US20240254511A1

公开(公告)日：2024-08-01

申请号：US18508761

申请日：2023-11-14

申请人： Whitehead Institute of Biomedical Research

发明人： Rudolf Jaenisch ,  Bryce Woodbury Carey ,  Yaqub Hanna

IPC分类号： C12N15/86 ,  A01K67/0271 ,  C07K14/47 ,  C12N5/074 ,  G01N33/50 ,  C12N5/0735 ,  C12N15/79 ,  C12N15/85

CPC分类号： C12N15/86 ,  A01K67/0271 ,  C07K14/4705 ,  C12N5/0696 ,  G01N33/5008 ,  C12N5/0606 ,  C12N15/79 ,  C12N15/85 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/11 ,  C12N2506/115 ,  C12N2510/00 ,  C12N2740/15041 ,  C12N2740/15043 ,  C12N2740/16043 ,  C12N2799/027 ,  C12N2800/108 ,  C12N2840/203

摘要： The disclosure relates to a method of reprogramming one or more somatic cells, e.g., partially differentiated or fully/terminally differentiated somatic cells, to a less differentiated state, e.g., a pluripotent or multipotent state. In further embodiments the invention also relates to reprogrammed somatic cells produced by methods of the invention, to chimeric animals comprising reprogrammed somatic cells of the invention, to uses of said cells, and to methods for identifying agents useful for reprogramming somatic cells.

公开(公告)号：US12031151B2

公开(公告)日：2024-07-09

申请号：US17578681

申请日：2022-01-19

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Reginald Smith ,  Sarah Nicoletti ,  Victor Shashilov ,  Hongxia Wang ,  Jikang Wu ,  Abdelqader Zamamiri

IPC分类号： C12N5/00 ,  C12N5/071 ,  C12N15/79

CPC分类号： C12N5/0018 ,  C12N5/0682 ,  C12N15/79 ,  C12N2500/32 ,  C12N2500/60

摘要： Methods of improving recombinant protein titer and cell titer in cell culture using cell culture media having reduced impurities are provided, and well as cell culture media having reduced impurities that can used for the production of a recombinant protein and cells with improved titer. The cell culture media having reduced impurities comprises a HEPES buffer, and the reduced impurities are HEPES related impurities. In certain aspects, methods and media improve protein titer, cell growth, and/or viable cell density.

公开(公告)号：US20240209359A1

公开(公告)日：2024-06-27

申请号：US18225531

申请日：2023-07-24

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Feng ZHANG ,  Naomi HABIB

IPC分类号： C12N15/113 ,  C12N9/22 ,  C12N15/10 ,  C12N15/63 ,  C12N15/79 ,  C12N15/90 ,  G16B20/00 ,  G16B20/20 ,  G16B20/30 ,  G16B20/50 ,  G16B30/00 ,  G16B30/10

CPC分类号： C12N15/113 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/63 ,  C12N15/907 ,  G16B20/00 ,  G16B20/20 ,  G16B20/30 ,  G16B20/50 ,  G16B30/10 ,  C12N15/79 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2320/11 ,  C12N2320/30 ,  C12N2750/14143 ,  G16B30/00

摘要： Disclosed are locational or positional methods concerning CRISPR-Cas systems, and apparatus therefor.

公开(公告)号：US11872290B2

公开(公告)日：2024-01-16

申请号：US17080205

申请日：2020-10-26

申请人： Ecole Polytechnique Federale De Lausanne (EPFL)

发明人： Julianne Aebischer ,  Bernard Schneider ,  Cylia Rochat

IPC分类号： A61K48/00 ,  C12N15/86 ,  C12N15/113 ,  C12N15/79

CPC分类号： A61K48/0058 ,  C12N15/113 ,  C12N15/79 ,  C12N15/86 ,  C12N2750/14141 ,  C12N2750/14143 ,  C12N2830/008 ,  C12N2830/20 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2830/50

摘要： The present invention relates to a bicistronic expression vector for silencing a gene specifically in astrocytes and neurons, comprising two expression cassettes comprising a first and a second silencer sequence, respectively, wherein the expression of said first silencer sequence within astrocytes is regulated by an astrocyte-specific promoter and the expression of said second silencer sequence within neurons is regulated by a neuron-specific promoter. In a preferred embodiment, said first and second silencer sequences are SOD1 silencer sequences. Pharmaceutical composition comprising said bicistronic vector and the use of the same in the treatment of motoneuron diseases are further described.

公开(公告)号：US20230348620A1

公开(公告)日：2023-11-02

申请号：US17999769

申请日：2021-05-28

申请人： VALL D´HEBRON INSTITUTE OF ONCOLOGY (VHIO) ,  INSTITUCIO CATALANA DE RECERCA I ESTUDIS AVANCATS

发明人： Joaquin ARRIBAS LOPEZ ,  Irene RIUS RUIZ ,  Macarena ROMAN ALONSO ,  Alena GROS VIDAL ,  Carlos Alberto FAJARDO CALDERON ,  Christian KLEIN ,  Ekkehard MOESSNER

IPC分类号： C07K14/725 ,  C07K16/32 ,  C07K14/705 ,  C07K14/73 ,  C12N15/79 ,  G01N33/563 ,  A61P35/00

CPC分类号： C07K16/32 ,  A61P35/00 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/70517 ,  C07K14/70521 ,  C12N15/79 ,  G01N33/563 ,  C07K2317/24 ,  C07K2317/53 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2319/03

摘要： The present invention relates to a chimeric antigen receptor (CAR) capable of targeting p95HER2-expressing cells. The invention also relates to a single-chain variable fragment (ScFv), to an antigen-binding domain and to an antibody or antibody fragment thereof capable of binding to the p95HER2 antigen. The invention also relates to a method of cancer diagnosis and to a pharmaceutical composition for use in a method of preventing or treating cancer comprising the CAR and/or the antigen-binding domain or the antibody of the invention.

公开(公告)号：US20230348545A1

公开(公告)日：2023-11-02

申请号：US18245687

申请日：2021-09-30

申请人： LONZA LTD

发明人： Brigitte GASSER ,  Jennifer STAUDACHER ,  Diethard MATTANOVICH

IPC分类号： C07K14/47 ,  C12N15/79

CPC分类号： C07K14/4705 ,  C12N15/79

摘要： A recombinant eukaryotic host cell expressing a gene of interest (GOI) which is engineered by genetic modifications to increase expression of two or more genes encoding translation initiation factors (TIF genes) of the messenger ribonucleoprotein (mRNP), compared to the host cell prior to said one or more genetic modifications, wherein said TIF genes comprise at least a gene encoding eIF4A and a gene encoding eIF4G, and wherein expression of at least one of said TIF genes is under transcriptional control of a promoter different from the promoter controlling expression of said GOI.

公开(公告)号：US20230323398A1

公开(公告)日：2023-10-12

申请号：US18160241

申请日：2023-01-26

申请人： Novartis AG

发明人： Chad Eric BIGELOW ,  Vivian CHOI ,  Thaddeus Peter DRYJA ,  Akshata Ninad GUJAR ,  Shawn Michael HANKS ,  Terri MCGEE ,  Seshidhar Reddy POLICE ,  Joanna VROUVLIANIS

IPC分类号： C12N15/86 ,  A61K48/00 ,  A61K38/17 ,  C07K14/47 ,  C12N7/00

CPC分类号： C12N15/86 ,  A61K38/1709 ,  A61K48/005 ,  A61K48/0058 ,  C07K14/47 ,  C12N7/00 ,  C12N15/79

摘要： The present invention relates to viral vectors that are capable of delivering a heterologous gene to the retina and in particular delivering RLBP1 to RPE and Müller cells of the retina. The invention also relates nucleic acids useful for producing viral vectors, compositions comprising the viral vectors and uses of the compositions and viral vectors. The invention also relates to methods of delivering and/or expressing a heterologous gene to the retina, improving the rate of dark adaption in a subject and treating RLBP1-associated retinal dystrophy.

公开(公告)号：US20230322866A1

公开(公告)日：2023-10-12

申请号：US17898870

申请日：2022-08-30

申请人： Suncor Energy Inc.

发明人： Samuel S. Slattery ,  Daniel J. Giguere ,  Martin Flatley ,  Gregory B. Gloor ,  David R. Edgell

IPC分类号： C12N15/79 ,  A61K39/215 ,  C07K14/165 ,  G01N33/543 ,  C12N1/12

CPC分类号： C07K14/165 ,  A61K39/215 ,  C12N1/12 ,  C12N15/79 ,  G01N33/54388 ,  C12N2500/24 ,  C12N2500/42

摘要： Phosphate-regulated expression of recombinant glycoprotein antigens and other recombinant proteins in diatoms is described herein. More specifically, described herein is the expression and purification of glycosylated, immunogenic, and serologically active receptor-binding domain (RBD) of the SARS-CoV-2 spike protein, as well as SARS-CoV-2 nucleocapsid protein, in the marine pennate diatom Phaeodactylum tricornutum, as well as a functional lateral flow assay-based diagnostic device based on the produced recombinant RBD and nucleocapsid protein. Also described herein is the use of phosphate/iron levels in culture media to regulate expression/secretion of recombinant proteins under control of an HASP1 promoter in P. tricornutum or other suitable host cells. Also described herein is a method for increasing the expression/secretion of a recombinant protein by engineering the recombinant protein to lack a Tobacco Etch Virus (TEV) protease cleavage site.