公开(公告)号：US20150368705A1

公开(公告)日：2015-12-24

申请号：US14763078

申请日：2014-01-22

Applicant: DYNAMIC BIOSENSORS GMBH

Inventor： Andreas LANGER ,  Ralf STRASSER ,  Ulrich RANT

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q2565/607

Abstract: The present invention is directed to sequencing of nucleic acids. A method is provided for sequencing based on immobilized nucleic acid on a surface. Advantageously, a long range detection mechanism is used for detecting, whether a nucleotide provided to the substrate of a biochip has been incorporated into the immobilized template nucleic acid. Various different alignment means are provided by the present invention which can be used for facilitating a rigidly locking of the orientation of the DNA complex, which complex comprises the template nucleic acid, the primer and the capture nucleic acid. Various different linker systems may be used to immobilize the DNA complex at a first and a second strand end, such that the desired alignment of the DNA complex is achieved. Also co-adsorbed molecules on the substrate surface can be used for such an aligning measure. Additionally, or alternatively, an electrical field may be applied for repelling the DNA complex from the electrode and for facilitating a vertical DNA complex orientation. Advantageously, label-free nucleotides can be used, if desired.

Abstract translation: 本发明涉及核酸的测序。 提供了一种基于表面上固定化核酸进行测序的方法。 有利地，远程检测机制用于检测提供给生物芯片的底物的核苷酸是否已经并入固定的模板核酸中。 通过本发明提供了各种不同的对准装置，其可用于促进DNA复合物的取向的刚性锁定，该复合物包含模板核酸，引物和捕获核酸。 可以使用各种不同的接头体系来固定DNA复合物在第一和第二链末端，从而实现DNA复合物的所需比对。 也可以将基底表面上的共吸附分子用于这种对准测量。 另外或替代地，可以施加电场以从电极排斥DNA复合物并促进垂直DNA复合物取向。 有利地，如果需要，可以使用无标记的核苷酸。