公开(公告)号：US07825294B2

公开(公告)日：2010-11-02

申请号：US12503952

申请日：2009-07-16

申请人： Daniel J. Altier ,  I. A. Ellanskaya ,  Jacob T. Gilliam ,  Jennie Hunter-Cevera ,  James K Presnail ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

发明人： Daniel J. Altier ,  I. A. Ellanskaya ,  Jacob T. Gilliam ,  Jennie Hunter-Cevera ,  James K Presnail ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/09 ,  C12N15/82

CPC分类号： C12N15/8282 ,  A01N37/46 ,  A01N63/04 ,  C07K14/37

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include an amino acid sequence, and variants and fragments thereof, for an antipathogenic polypeptide that was isolated from a fungal fermentation broth. Nucleic acid molecules that encode the antipathogenic polypeptides of the invention, and antipathogenic domains thereof, are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从真菌发酵液中分离的抗病原体多肽的氨基酸序列及其变体和片段。 还提供了编码本发明的抗病原体多肽的核酸分子及其抗病原结构域。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列的转化植物，植物细胞，种子和微生物。