公开(公告)号：US20150079660A1

公开(公告)日：2015-03-19

申请号：US14494436

申请日：2014-09-23

Applicant: DANISCO US INC.

Inventor： Vivek Sharma ,  Jayarama Shetty ,  Bruce Strohm

IPC: C07H1/08 ,  C12N9/28

CPC classification number: C07H1/08 ,  C12N9/2417 ,  C12P19/14

Abstract: The present disclosure relates to a Bacillus subtilis alpha-amylase (AmyE) or its variant thereof. AmyE or its variants thereof may be used to eliminate or reduce the iodine-positive starch presented in saccharide liquor. Also disclosed are a composition comprising an AmyE or variant thereof and a method utilizing an AmyE or variant thereof to eliminate or reduce the iodine-positive starch

Abstract translation: 本公开涉及枯草芽孢杆菌α-淀粉酶（AmyE）或其变体。 可以使用AmyE或其变体来消除或减少糖液中存在的碘阳性淀粉。 还公开了包含AmyE或其变体的组合物和利用其AmyE或其变体来消除或减少碘阳性淀粉的方法

公开(公告)号：US20130288309A1

公开(公告)日：2013-10-31

申请号：US13857004

申请日：2013-04-04

Applicant: Danisco US Inc.

Inventor： Gang DUAN ,  Sung Ho Lee ,  Ying Qian ,  Rafael F. Sala ,  Jayarama Shetty

IPC: C12P19/14 ,  C12P19/16 ,  C12P7/14

CPC classification number: C12P19/14 ,  C12N9/2417 ,  C12P7/10 ,  C12P7/14 ,  C12P19/16 ,  D06L1/14 ,  D06M16/003 ,  Y02E50/16

Abstract: Variants of a Pseudomonas saccharophila G4-forming amylase (PS4) advantageously can catalyze high temperature saccharification to produce maltotetraose syrup from a starch liquefact or granular starch, e.g., derived from cornstarch. The PS4 variants are useful in a process of saccharification of starch that advantageously produces significant amounts of maltotetraose, which can be used downstream in a process of producing a maltotetraose syrup. In one embodiment, a thermostable PS4 variant is provided that can produce about 40% to about 60% by weight maltotetraose, based on total saccharide content.

Abstract translation: 酿酒酵母G4形成淀粉酶（PS4）的变体有利地可以催化高温糖化以从淀粉液化或颗粒淀粉（例如衍生自玉米淀粉）产生麦芽四糖糖浆。 PS4变体可用于淀粉糖化过程，其有利地产生显着量的麦芽四糖，其可以在生产麦芽四糖糖浆的方法中在下游使用。 在一个实施方案中，提供了基于总糖含量可产生约40重量％至约60重量％麦芽四糖的耐热性PS4变体。