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公开(公告)号:US5008081A
公开(公告)日:1991-04-16
申请号:US256022
申请日:1988-10-11
申请人: David A. Blau , John Meadows , Thomas M. Sherlock , Fred Stengel , Robert M. Studholme , Kenneth R. Wada , Christopher J. Kepner
发明人: David A. Blau , John Meadows , Thomas M. Sherlock , Fred Stengel , Robert M. Studholme , Kenneth R. Wada , Christopher J. Kepner
CPC分类号: G01N33/442 , G01N11/04 , G01N35/00 , Y10T436/11
摘要: Computer-controlled method and apparatus for automatic melt index testing, including a housing with a base carrying a heater block having a bore therein for receiving a metal cartridge. A cartridge carousel is rotatably carried on the base, and carries a plurality of such cartridges, each cartridge having a plug at the bottom end thereof with a orifice through the plug, and each cartridge having a bore therein for receiving a sample of a test substance and for receiving a piston on top of the pellets. A lift arm grasps the cartridges, one at a time, and places each cartridge into the heater block, under the control of a microcomputer, by both lifting and rotating the transfer arm. The transfer arm includes a solenoid-operated grip for grasping the cartridges.The test unit carries a lift mechanism with a weight mounted atop a tamping rod, and an optical sensor for determining the height of the weight relative to the lift machinism. The weight is lowered by the lifting mechanism until it bears down upon the pellets, and then the cartridge is heated until the test substance melts. The weight is then used to extrude the melted substance through the plug orifice until it has fallen a certain distance, which operation is automatically timed by the computer. The time is then used in determining the melt index value for the substance. This operation is automatically repeated for all of the cartridges in the carousel.An automatic cut-off mechanism is used to cut off excess extrudate, and an extrudate carousel is automatically advanced to collect separate samples of the used extrudate. Optical sensors are provided to determine the motions of the components of the apparatus.
摘要翻译: 用于自动熔融指数测试的计算机控制方法和装置,包括具有承载加热器块的底座的壳体,其中具有用于容纳金属盒的孔。 筒式转盘可旋转地承载在基座上,并且承载多个这样的盒,每个盒的底端具有一个插塞,其中一个孔通过插头,每个盒都有一个孔,用于接收一个测试物质的样本 并且用于在颗粒的顶部上接收活塞。 提升臂通过提升和旋转传送臂,一次一个握住盒式磁带,并在微型计算机的控制下将每个盒放置在加热器块中。 传送臂包括用于抓住盒的电磁操作把手。 测试单元携带一个重量安装在夯实杆上的升降机构,以及用于确定重量相对于提升机械加工度的高度的光学传感器。 通过提升机构降低重量,直到它压在丸粒上,然后加热筒直到被测物质熔化。 然后重量用于通过塞孔挤出熔化的物质,直到其下降一定距离,该操作由计算机自动计时。 然后将时间用于确定物质的熔体指数值。 旋转木马盘中的所有墨盒都会自动重复此操作。 自动切断机构用于切断多余的挤出物,并自动推进挤出物转盘以收集所使用的挤出物的单独样品。 提供光学传感器以确定装置的部件的运动。
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公开(公告)号:US07354704B2
公开(公告)日:2008-04-08
申请号:US10284053
申请日:2002-10-29
CPC分类号: G01N33/5008 , B01J19/0046 , B01J2219/00317 , B01J2219/00605 , B01J2219/0061 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00713 , B01L3/5085 , C40B60/14 , G01N33/502
摘要: High-throughput screening method and apparatus arm described. The method includes placing cells on a substrate defining a plurality of discrete microwells, at a well density of greater than about 100/cm2, with the number of cells in each well being less than about 1000, and where the cells in each well have been exposed to a selected agent. The change in conductance in each well is determined by applying a low-voltage, AC signal across a pair of electrodes placed in that well, and synchronously measuring the conductance across the electrodes, to monitor the level of growth or metabolic activity of cells contained in each well. Also disclosed is an apparatus for carrying out the screening method.
摘要翻译: 描述高通量筛选方法和装置臂。 该方法包括将细胞置于限定多个离散微孔的基底上,孔密度大于约100 / cm 2,每个孔中的细胞数小于约1000,以及 其中每个孔中的细胞已经暴露于选定的试剂。 通过在放置在该孔中的一对电极上施加低电压AC信号,同步测量电极两端的电导,来监测每个孔中电导的变化,以监测包含在细胞中的细胞的生长或代谢活性水平 每个井 还公开了一种用于进行筛选方法的装置。
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公开(公告)号:US06472144B2
公开(公告)日:2002-10-29
申请号:US09813922
申请日:2001-03-20
IPC分类号: C12Q100
CPC分类号: G01N33/5008 , B01J19/0046 , B01J2219/00317 , B01J2219/00605 , B01J2219/0061 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00713 , B01L3/5085 , C40B60/14 , G01N33/502
摘要: High-throughput screening method and apparatus are described. The method includes placing cells on a substrate defining a plurality of discrete microwells, at a well density of greater than about 100/cm2, with the number of cells in each well being less than about 1000, and where the cells in each well have been exposed to a selected agent. The change in conductance in each well is determined by applying a low-voltage, AC signal across a pair of electrodes paced in that well, and synchronously measuring the conductance across the electrodes, to monitor the level of growth or metabolic activity of cells contained in each well. Also disclosed is an apparatus for carrying out the screening method.
摘要翻译: 描述了高通量筛选方法和装置。 该方法包括将细胞置于限定多个离散微孔的基底上,孔密度大于约100 / cm2,每个孔中的细胞数小于约1000,并且其中每个孔中的细胞已经被 暴露于所选择的代理。 每个孔中的电导率的变化是通过将一个低电压的交流信号施加在该孔中的一对电极上,同时测量电极两端的电导,来监测包含在电极中的细胞的生长或代谢活动的水平 每个井 还公开了一种用于进行筛选方法的装置。
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公开(公告)号:US06235520B1
公开(公告)日:2001-05-22
申请号:US09202658
申请日:1999-05-14
IPC分类号: C12M134
CPC分类号: G01N33/5008 , B01J19/0046 , B01J2219/00317 , B01J2219/00605 , B01J2219/0061 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00713 , B01L3/5085 , C40B60/14 , G01N33/502
摘要: High-throughout screening method and apparatus are described. The method includes placing cells on a substrate defining a plurality of discrete microwells, at a well density of greater than about 100/cm2, with the number of sells in each well being less that about 1000, and where the cells in each well have been exposed to a selected agent. The change in conductance in each well is determined by applying a low-voltage, AC signal across a pair of electrodes placed in that well, and synchronously measuring the conductance across the electrodes, to monitor the level of growth or metabolic activity of cells contained in each well. Also disclosed is an apparatus for carrying out the screening method.
摘要翻译: 描述了高整体的筛选方法和装置。 该方法包括将细胞置于限定多个离散微孔的衬底上,孔密度大于约100 / cm 2,每个孔中的销售数量小于约1000,并且每个孔中的细胞已经被 暴露于所选择的代理。 通过在放置在该孔中的一对电极上施加低电压AC信号,同步测量电极两端的电导,来监测每个孔中电导的变化,以监测包含在细胞中的细胞的生长或代谢活性水平 每个井 还公开了一种用于进行筛选方法的装置。
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