公开(公告)号：US06566469B1

公开(公告)日：2003-05-20

申请号：US09602649

申请日：2000-06-22

申请人： David Kaplan ,  Graham Swift ,  Richard A. Gross ,  Bhanu Kalra

发明人： David Kaplan ,  Graham Swift ,  Richard A. Gross ,  Bhanu Kalra

IPC分类号： C08F430

CPC分类号： C12P1/00

摘要： A method for polymerization of substituted ethylene monomers in an enzyme-mediated process is disclosed. The reaction proceeds under mild conditions with a wide variety of monomers, and is capable of producing stereoregular polymers. In certain embodiments, no organic solvent is necessary.

摘要翻译： 公开了在酶介导的方法中聚合取代的乙烯单体的方法。 该反应在温和条件下与各种各样的单体进行，并且能够产生立体有规聚合物。 在某些实施方案中，不需要有机溶剂。

公开(公告)号：US06972315B2

公开(公告)日：2005-12-06

申请号：US10199434

申请日：2002-07-19

申请人： Richard A. Gross ,  Ajay Kumar ,  Bhanu Kalra

发明人： Richard A. Gross ,  Ajay Kumar ,  Bhanu Kalra

IPC分类号： C08G63/78 ,  C08G63/87 ,  C08G63/02

CPC分类号： C08G63/87 ,  C08G63/78

摘要： A method for enzymatic condensation polymerization by combining a preselected quantity of an enzyme, a diol and/or a polyol, and a diacid in a reaction vessel; heating the reaction vessel containing the enzyme, the diol or polyol, and the diacid to a preselected temperature; and maintaining the reaction vessel containing the enzyme, the diol and/or polyol, and the diacid at the preselected temperature for a preselected time, thereby producing a condensation polymer.

摘要翻译： 一种通过将预选量的酶，二醇和/或多元醇与二酸在反应容器中组合来进行酶缩缩聚合的方法; 将含有酶，二醇或多元醇的反应容器和二酸加热至预选温度; 并在预选的温度下保持含有酶，二醇和/或多元醇和二酸的反应容器预选的时间，从而制备缩聚物。

公开(公告)号：US20050272108A1

公开(公告)日：2005-12-08

申请号：US11134064

申请日：2005-05-20

申请人： Bhanu Kalra ,  Ajay Kumar

发明人： Bhanu Kalra ,  Ajay Kumar

IPC分类号： G01N33/53 ,  G01N33/533 ,  G01N33/537 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N33/533 ,  G01N33/582

摘要： The present invention provides stabilized chemiluminescent formulations for use in in vitro diagnostics, including competitive as well as sandwich-type immunological assays. The stabilized assay system may be composed of two components, where the first component may contain a chemiluminescent organic compound, an enhancer, a homogenizing agent, and a suitable buffer with formulations having a pH range from about 7.2 to about 12, and optionally a solubilizing agent. The chemiluminescent system of the present invention is useful in immunoenzymatic analytical procedures, such as immunometric, competitive binding and sandwich type assays. In such immunoassays employing the chemiluminescent system of the present invention, the detectable light signal shows a proportional decay with time in the test samples and standards, so that the decay of the light emitted does not effect the concentration of the analyte measured over the entire analyte measurement range of the immunoassay. This allows accurate measurement of analyte concentrations in a test sample over extended periods of time.

摘要翻译： 本发明提供用于体外诊断的稳定的化学发光制剂，包括竞争性和夹心型免疫测定。 稳定化的测定系统可以由两个组分组成，其中第一组分可以含有化学发光有机化合物，增强剂，均化剂和具有约7.2至约12的pH范围的制剂的合适缓冲液，以及任选的增溶 代理商 本发明的化学发光系统可用于免疫酶学分析程序，例如免疫测定，竞争性结合和夹心型测定。 在采用本发明的化学发光系统的这种免疫测定中，可检测的光信号在测试样品和标准品中显示随时间的比例衰减，使得发射的光的衰变不影响在整个分析物上测量的分析物的浓度 测量范围的免疫测定。 这允许在长时间内精确测量测试样品中的分析物浓度。