METHOD FOR CULTURING BACILLUS BACTERIUM, AND METHOD FOR PRODUCING USEFUL SUBSTANCE

    公开(公告)号:US20190241924A1

    公开(公告)日:2019-08-08

    申请号:US16339861

    申请日:2017-10-06

    发明人: Yuki TSUKAGOSHI

    IPC分类号: C12P21/00 C12N1/20

    CPC分类号: C12P21/00 C12N1/20 C12P1/00

    摘要: An objective of the present invention is to provide a culturing method, by which Bacillus bacterial spores can be efficiently produced. The present invention provides a method for producing Bacillus bacterial spores, comprising culturing Bacillus bacteria in a liquid medium containing a sugar or a sugar-source raw material at a concentration between 50.1 g/L and 100 g/L at the start of culture, and then in the course of the culture, feeding a fed-batch medium containing a sugar or a sugar-source raw material and a nitrogen-containing compound, and having the weight ratio (C/N ratio) of carbon atoms to nitrogen atoms ranging from 5.5 to 13.5 to the liquid medium.

    METHOD OF PRODUCING A CHEMICAL PRODUCT
    8.
    发明申请
    METHOD OF PRODUCING A CHEMICAL PRODUCT 审中-公开
    生产化学产品的方法

    公开(公告)号:US20160168601A1

    公开(公告)日:2016-06-16

    申请号:US15051710

    申请日:2016-02-24

    IPC分类号: C12P7/56

    摘要: A method of producing a chemical product includes cultivating microorganisms or culture cells in a fermentation tank; transferring a culture liquid from the fermentation tank to a membrane separation tank to filter the culture liquid through a separation membrane; and collecting a fermentation product from a filtration liquid as the chemical product while refluxing an unfiltered culture liquid that has not been filtered to be joined to the culture liquid on an upstream side of the membrane separation tank, wherein one portion of the culture liquid to be transferred from the fermentation tank is allowed to bypass the membrane separation tank depending on a pressure at a culture liquid flow-in side of the membrane separation tank.

    摘要翻译: 生产化学产品的方法包括在发酵罐中培养微生物或培养细胞; 将培养液从发酵罐转移到膜分离罐中,通过分离膜过滤培养液; 并从作为化学产品的过滤液中收集发酵产物,同时在膜分离罐的上游侧回流未过滤的待过滤培养液与培养液接合的培养液,其中一部分培养液为 从膜分离罐的培养液流入侧的压力,允许从发酵罐转移到膜分离槽旁路。

    Microvesicles
    9.
    发明申请
    Microvesicles 审中-公开
    微泡

    公开(公告)号:US20160032322A1

    公开(公告)日:2016-02-04

    申请号:US14789121

    申请日:2015-07-01

    发明人: Stephen Ray

    IPC分类号: C12P1/00 C12N5/071

    摘要: A method of producing a population of differentiated cells comprising: a) inducing differentiation in a first population of cells by applying an inducer to said cells, b) harvesting microvesicles produced from first population of cells, and c) inducing differentiation in a second population of cells by applying said microvesicles or a derivative thereof to said second population of cells wherein, said first population of cells is autologous to said second population of cells and wherein the inducer applied to said first population of cells is not present in said second population of cells or is only present in trace amounts. Also related methods of producing microvesicles, methods of medical and/or cosmetic treatment and related products and uses.

    摘要翻译: 一种产生分化细胞群体的方法,包括:a)通过向所述细胞施加诱导剂来诱导第一群细胞中的分化,b)收获由第一群细胞产生的微泡,以及c)在第二群体中诱导分化 通过将所述微泡或其衍生物施用于所述第二细胞群,其中所述第一细胞群与所述第二细胞群自体,并且其中应用于所述第一细胞群的诱导物不存在于所述第二细胞群中 或仅存在痕量。 还涉及生产微泡的方法,医疗和/或美容治疗方法以及相关产品和用途。

    METHOD TO OPTIMIZE THE UTILIZATION OF CAPTURED CARBON DIOXIDE THROUGH THE CULTIVATION AND PROCESSING OF MICROALGAE
    10.
    发明申请
    METHOD TO OPTIMIZE THE UTILIZATION OF CAPTURED CARBON DIOXIDE THROUGH THE CULTIVATION AND PROCESSING OF MICROALGAE 审中-公开
    通过微生物加工和加工优化捕获的二氧化碳的方法

    公开(公告)号:US20150307830A1

    公开(公告)日:2015-10-29

    申请号:US14261854

    申请日:2014-04-25

    IPC分类号: C12M1/00 C12P1/00 C12P3/00

    摘要: A method for cultivating and processing microalgae includes directing a continuous supply of carbon dioxide into an enclosed photobioreactor containing microalgae. The enclosed photobioreactor is exposed to insolation for the cultivation of the microalgae by means of controlled photosynthesis chemical reactions. The microalgae are cultivated and the microalgae are processed to create an algae biomass and a gaseous mixture. The algae biomass is extracted to produce an essentially pure algae oil and oil free algae biomass. The gaseous mixture is separated into unreacted carbon dioxide and oxygen. The separated unreacted carbon dioxide is recycled to the photobioreactor and the oxygen in excess of export quantity is stored.

    摘要翻译: 微藻的培养和加工方法包括将二氧化碳的连续供应引导到包含微藻的封闭的光生物反应器中。 封闭的光生物反应器通过受控的光合作用化学反应暴露于日晒以培养微藻。 培养微藻,并处理微藻以产生藻类生物质和气体混合物。 提取藻类生物质以产生基本上纯的藻类油和无油藻类生物质。 将气体混合物分离成未反应的二氧化碳和氧气。 将分离的未反应的二氧化碳再循环到光生物反应器中,并且存储超过出口量的氧气。