公开(公告)号：US20110172394A1

公开(公告)日：2011-07-14

申请号：US13001287

申请日：2009-06-24

申请人： David Knight ,  Nicholas Skaer ,  Andrew Collins ,  Tom Louis Dirk Gheysens

发明人： David Knight ,  Nicholas Skaer ,  Andrew Collins ,  Tom Louis Dirk Gheysens

IPC分类号： C07K14/435

CPC分类号： A61L27/46 ,  A61L27/3604 ,  A61L27/365 ,  A61L27/56 ,  A61L2430/02

摘要： A method for the preparation of an implantable material for the repair, augmentation or replacement of bone from a fibroin solution, the method comprising the steps of: preparing a gel from the fibroin solution; preparing a material by subjecting the gel to one or more steps of freezing and thawing the gel, wherein the step of preparing the gel from the fibroin solution is performed in the presence of phosphate ions. The material may be treated with calcium ionstoform a fibroin-apatite. A further method comprises the step of treating the material with an isocyanate. The invention also extends to a method for the preparation of an implantable material, wherein a regenerated fibroin solution is used. Also, there is an implantable material and an implant.

摘要翻译： 一种用于制备用于从丝心蛋白溶液修复，增加或替换骨的可植入材料的方法，所述方法包括以下步骤：从丝心蛋白溶液制备凝胶; 通过使凝胶进行一个或多个步骤来冷冻和解冻凝胶来制备材料，其中在磷酸盐离子存在下从丝心蛋白溶液制备凝胶的步骤。 该材料可以用离子交换丝心蛋白磷灰石来处理。 另一种方法包括用异氰酸酯处理该材料的步骤。 本发明还延伸到制备可植入材料的方法，其中使用再生的丝心蛋白溶液。 此外，还有可植入材料和植入物。

公开(公告)号：US20120142898A1

公开(公告)日：2012-06-07

申请号：US13368707

申请日：2012-02-08

申请人： David Philip Knight ,  Nicholas James Vavasour Skaer ,  Andrew Michael Collins ,  Tom Louis Dirk Gheysens ,  Christopher Holland

发明人： David Philip Knight ,  Nicholas James Vavasour Skaer ,  Andrew Michael Collins ,  Tom Louis Dirk Gheysens ,  Christopher Holland

IPC分类号： C07K1/02

CPC分类号： A61L27/3687 ,  A61L27/3604 ,  C08H1/00 ,  C08L89/00

摘要： A method for the preparation of a regenerated silk fibroin solution comprises the steps of: treating silk or silk cocoons with an ionic reagent comprising an aqueous solution of monovalent cations and monovalent anions, the cations and anions having ionic radii of at least 1.05 Angstroms and a Jones-Dole B coefficient of between −0.001 and −0.05 at 25° C.; and subsequently degumming the treated silk or silk cocoons; or alternatively, degumming silk or silk cocoons; and subsequently treating the degummed silk or silk cocoons with an ionic reagent comprising an aqueous solution of monovalent cations and monovalent anions, the cations and anions having ionic radii of at least 1.05 Angstroms and a Jones-Dole B coefficient of between −0.001 and −0.05 at 25° C. The invention also extends to fibroin solution, a fibroin material and an implant useful for cartilage repair.

摘要翻译： 一种制备再生丝素蛋白溶液的方法包括以下步骤：用包含一价阳离子和一价阴离子的水溶液的离子试剂处理丝或丝茧，所述阳离子和阴离子的离子半径至少为1.05埃， Jones-Dole B系数在25℃在-0.001和-0.05之间。 然后使经处理的丝或丝茧脱胶; 或者脱胶丝或丝茧; 并随后用包含一价阳离子和一价阴离子水溶液的离子试剂处理脱胶的丝或丝茧，阳离子和阴离子的离子半径至少为1.05埃，琼斯 - 多尔B系数在-0.001-0.05之间 在25℃下。本发明还延伸到丝心蛋白溶液，丝心蛋白材料和用于软骨修复的植入物。

公开(公告)号：US08337938B2

公开(公告)日：2012-12-25

申请号：US13368707

申请日：2012-02-08

申请人： David Philip Knight ,  Nicholas James Vavasour Skaer ,  Andrew Michael Collins ,  Tom Louis Dirk Gheysens ,  Christopher Holland

发明人： David Philip Knight ,  Nicholas James Vavasour Skaer ,  Andrew Michael Collins ,  Tom Louis Dirk Gheysens ,  Christopher Holland

IPC分类号： A61K6/083

CPC分类号： A61L27/3687 ,  A61L27/3604 ,  C08H1/00 ,  C08L89/00

摘要： A method for the preparation of a regenerated silk fibroin solution comprises the steps of: treating silk or silk cocoons with an ionic reagent comprising an aqueous solution of monovalent cations and monovalent anions, the cations and anions having ionic radii of at least 1.05 Angstroms and a Jones-Dole B coefficient of between −0.001 and −0.05 at 25° C.; and subsequently degumming the treated silk or silk cocoons; or alternatively, degumming silk or silk cocoons; and subsequently treating the degummed silk or silk cocoons with an ionic reagent comprising an aqueous solution of monovalent cations and monovalent anions, the cations and anions having ionic radii of at least 1.05 Angstroms and a Jones-Dole B coefficient of between −0.001 and −0.05 at 25° C. The invention also extends to fibroin solution, a fibroin material and an implant useful for cartilage repair.

公开(公告)号：US08128984B2

公开(公告)日：2012-03-06

申请号：US12990399

申请日：2009-04-30

申请人： David Philip Knight ,  Nicholas James Vavasour Skaer ,  Andrew Michael Collins ,  Tom Louis Dirk Gheysens ,  Christopher Holland

发明人： David Philip Knight ,  Nicholas James Vavasour Skaer ,  Andrew Michael Collins ,  Tom Louis Dirk Gheysens ,  Christopher Holland

IPC分类号： A61K6/083

CPC分类号： A61L27/3687 ,  A61L27/3604 ,  C08H1/00 ,  C08L89/00

摘要： A method for the preparation of a regenerated silk fibroin solution comprises the steps of: treating silk or silk cocoons with an ionic reagent comprising an aqueous solution of monovalent cations and monovalent anions, the cations and anions having ionic radii of at least 1.05 Angstroms and a Jones-Dole B coefficient of between −0.001 and −0.05 at 25° C.; and subsequently degumming the treated silk or silk cocoons; or alternatively, degumming silk or silk cocoons; and subsequently treating the degummed silk or silk cocoons with an ionic reagent comprising an aqueous solution of monovalent cations and monovalent anions, the cations and anions having ionic radii of at least 1.05 Angstroms and a Jones-Dole B coefficient of between −0.001 and −0.05 at 25° C. The invention also extends to fibroin solution, a fibroin material and an implant useful for cartilage repair.

摘要翻译： 一种制备再生丝素蛋白溶液的方法包括以下步骤：用包含一价阳离子和一价阴离子的水溶液的离子试剂处理丝或丝茧，所述阳离子和阴离子的离子半径至少为1.05埃， Jones-Dole B系数在25℃在-0.001和-0.05之间。 然后使经处理的丝或丝茧脱胶; 或者脱胶丝或丝茧; 并随后用包含一价阳离子和一价阴离子水溶液的离子试剂处理脱胶的丝或丝茧，阳离子和阴离子的离子半径至少为1.05埃，琼斯 - 多尔B系数在-0.001-0.05之间 在25℃下。本发明还延伸到丝心蛋白溶液，丝心蛋白材料和用于软骨修复的植入物。

公开(公告)号：US20110177151A1

公开(公告)日：2011-07-21

申请号：US12990399

申请日：2009-04-30

申请人： David Philip Knight ,  Nicholas James Vavasour Skaer ,  Andrew Michael Collins ,  Tom Louis Dirk Gheysens ,  Christopher Holland

发明人： David Philip Knight ,  Nicholas James Vavasour Skaer ,  Andrew Michael Collins ,  Tom Louis Dirk Gheysens ,  Christopher Holland

IPC分类号： A61K38/17 ,  C12S3/14 ,  C07K1/00 ,  A61K9/00 ,  C07K14/435 ,  A61P19/04 ,  A61P19/00

CPC分类号： A61L27/3687 ,  A61L27/3604 ,  C08H1/00 ,  C08L89/00

摘要： A method for the preparation of a regenerated silk fibroin solution comprises the steps of: treating silk or silk cocoons with an ionic reagent comprising an aqueous solution of monovalent cations and monovalent anions, the cations and anions having ionic radii of at least 1.05 Angstroms and a Jones-Dole B coefficient of between −0.001 and −0.05 at 25° C.; and subsequently degumming the treated silk or silk cocoons; or alternatively, degumming silk or silk cocoons; and subsequently treating the degummed silk or silk cocoons with an ionic reagent comprising an aqueous solution of monovalent cations and monovalent anions, the cations and anions having ionic radii of at least 1.05 Angstroms and a Jones-Dole B coefficient of between −0.001 and −0.05 at 25° C. The invention also extends to fibroin solution, a fibroin material and an implant useful for cartilage repair.

摘要翻译： 一种制备再生丝素蛋白溶液的方法包括以下步骤：用包含一价阳离子和一价阴离子的水溶液的离子试剂处理丝或丝茧，所述阳离子和阴离子的离子半径至少为1.05埃， Jones-Dole B系数在25℃在-0.001和-0.05之间。 然后使经处理的丝或丝茧脱胶; 或者脱胶丝或丝茧; 并随后用包含一价阳离子和一价阴离子水溶液的离子试剂处理脱胶的丝或丝茧，阳离子和阴离子的离子半径至少为1.05埃，琼斯 - 多尔B系数在-0.001-0.05之间 在25℃下。本发明还延伸到丝心蛋白溶液，丝心蛋白材料和用于软骨修复的植入物。