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公开(公告)号：US07569357B2
公开(公告)日：2009-08-04
申请号：US10783786
申请日：2004-02-20
IPC分类号： G01N33/563 , G01N33/53
CPC分类号： C40B40/02 , C07K14/7051 , C12N15/1037 , G01N33/68 , G01N33/6842 , G01N33/6854 , G01N2333/39
摘要： T cell receptors (TCRS) that have higher affinity for a ligand than wild type TCRs are provided. These high affinity TCRs are formed by mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants of the T cell receptor protein coding sequence; transforming the T cell receptor mutant coding sequence into yeast cells; inducing expression of the T cell receptor mutant coding sequence on the surface of yeast cells; and selecting those cells expressing T cell receptor mutants that have higher affinity for the peptide/MHC ligand than the wild type T cell receptor protein. The high affinity TCRs can be used in place of an antibody or single chain antibody.
摘要翻译： 提供了比野生型TCR对配体具有更高亲和力的T细胞受体（TCRS）。 通过诱变T细胞受体蛋白质编码序列产生T细胞受体蛋白编码序列突变体的杂色群体，形成这些高亲和性TCR。 将T细胞受体突变体编码序列转化为酵母细胞; 在酵母细胞表面诱导T细胞受体突变体编码序列的表达; 并选择那些表达与野生型T细胞受体蛋白相比对肽/ MHC配体具有更高亲和力的T细胞受体突变体的细胞。 高亲和性TCR可用于代替抗体或单链抗体。
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公开(公告)号：US06759243B2
公开(公告)日：2004-07-06
申请号：US09731242
申请日：2000-12-06
IPC分类号： C12N510
CPC分类号： C40B40/02 , C07K14/7051 , C12N15/1037 , G01N33/68 , G01N33/6842 , G01N33/6854 , G01N2333/39
摘要： T cell receptors (TCRs) that have higher affinity for a ligand than wild type TCRs are provided. These high affinity TCRs are formed by mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants of the T cell receptor protein coding sequence; transforming the T cell receptor mutant coding sequence into yeast cells; inducing expression of the T cell receptor mutant coding sequence on the surface of yeast cells; and selecting those cells expressing T cell receptor mutants that have higher affinity for the peptide/MHC ligand than the wild type T cell receptor protein. The high affinity TCRs can be used in place of an antibody or single chain antibody.
摘要翻译： 提供了比野生型TCR对配体具有更高亲和力的T细胞受体（TCR）。 通过诱变T细胞受体蛋白质编码序列产生T细胞受体蛋白编码序列突变体的杂色群体，形成这些高亲和性TCR。 将T细胞受体突变体编码序列转化为酵母细胞; 在酵母细胞表面诱导T细胞受体突变体编码序列的表达; 并选择那些表达与野生型T细胞受体蛋白相比对肽/ MHC配体具有更高亲和力的T细胞受体突变体的细胞。 高亲和性TCR可用于代替抗体或单链抗体。
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公开(公告)号：US20090275137A1
公开(公告)日：2009-11-05
申请号：US12494458
申请日：2009-06-30
CPC分类号： C07K14/7051 , C12N5/0636 , C12N15/1037 , C12N2510/00 , C40B40/02 , G01N33/56972 , G01N33/68 , G01N33/6842 , G01N33/6854 , G01N2333/39 , G01N2333/7051
摘要： T cell receptors (TCRS) that have higher affinity for a ligand than wild type TCRs are provided. These high affinity TCRs are formed by mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants of the T cell receptor protein coding sequence; transforming the T cell receptor mutant coding sequence into yeast cells; inducing expression of the T cell receptor mutant coding sequence on the surface of yeast cells; and selecting those cells expressing T cell receptor mutants that have higher affinity for the peptide/MHC ligand than the wild type T cell receptor protein. The high affinity TCRs can be used in place of an antibody or single chain antibody.
摘要翻译： 提供了比野生型TCR对配体具有更高亲和力的T细胞受体（TCRS）。 通过诱变T细胞受体蛋白质编码序列产生T细胞受体蛋白编码序列突变体的杂色群体，形成这些高亲和性TCR。 将T细胞受体突变体编码序列转化为酵母细胞; 在酵母细胞表面诱导T细胞受体突变体编码序列的表达; 并选择那些表达与野生型T细胞受体蛋白相比对肽/ MHC配体具有更高亲和力的T细胞受体突变体的细胞。 高亲和性TCR可用于代替抗体或单链抗体。
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