BACTERIOPHAGE IMMOBILIZATION FOR BIOSENSORS
    2.
    发明申请
    BACTERIOPHAGE IMMOBILIZATION FOR BIOSENSORS 审中-公开
    生物传感器的细菌保护

    公开(公告)号:US20100291541A1

    公开(公告)日:2010-11-18

    申请号:US12466089

    申请日:2009-05-14

    IPC分类号: C12Q1/70 C12N7/00 C12M1/34

    摘要: A method is disclosed for anchoring a bacteriophage on a substrate, the bacteriophage having a phage amine moiety, the method comprising: producing a free amine terminal moiety on the substrate by chemical modification of the substrate; activating the free amine terminal moiety with a cross-linking agent to produce an active functional group to couple to the phage amine moiety; and anchoring the bacteriophage to the substrate using the active functional group. A sensor is also disclosed comprising: a substrate; an anchor group attached by chemical modification to the substrate and having an active functional group produced by the activation of a free amine terminal moiety; and a bacteriophage having a phage amine moiety coupled to the active functional group to anchor the bacteriophage to the substrate.

    摘要翻译: 公开了一种用于将噬菌体锚定在基底上的方法,该噬菌体具有噬菌体胺部分,该方法包括:通过基底的化学修饰在基底上产生游离胺末端部分; 用交联剂活化游离胺末端部分以产生与噬菌体胺部分偶联的活性官能团; 并使用活性官能团将噬菌体锚定到底物上。 还公开了一种传感器,包括:基底; 通过化学修饰连接到底物并具有通过游离胺末端部分的活化产生的活性官能团的锚定基团; 以及噬菌体,其具有与活性官能团偶联的噬菌体胺部分以将噬菌体锚定于底物。