公开(公告)号：US5510894A

公开(公告)日：1996-04-23

申请号：US351175

申请日：1994-11-30

Applicant: David N. Batchelder ,  G. David Pitt

Inventor： David N. Batchelder ,  G. David Pitt

IPC: G01J3/12 ,  G01J3/28 ,  G01J3/44 ,  G01N21/65 ,  G02B21/00 ,  G01F3/34

CPC classification number: G01J3/02 ,  B82Y15/00 ,  G01J3/0208 ,  G01J3/04 ,  G01J3/2803 ,  G01J3/44 ,  G01N21/65 ,  G02B21/004 ,  G02B21/0064 ,  G02B21/008 ,  G01J2003/1226 ,  G01N2021/1772 ,  G01N2021/1782 ,  G01N2021/656

Abstract: A spot of a sample is illuminated by laser light. Raman scattered light is collimated in a parallel beam by a microscope objective, and analyzed by a dispersive or non-dispersive analyzer (such as a diffraction grating or filter). A lens then focuses the Raman scattered light onto a two-dimensional photodetector array in the form of a charge-coupled device (CCD). A confocal technique is described to eliminate light scattered from outside the focal plane of the objective. This may be done by binning together a few pixels of the CCD at the focal point of the lens, or by image processing techniques in a computer.

Abstract translation: 样品点由激光照亮。 拉曼散射光通过显微镜物镜在平行光束中准直，并通过分散或非色散分析仪（例如衍射光栅或滤光片）进行分析。 然后，透镜将拉曼散射光聚焦成电荷耦合器件（CCD）形式的二维光电检测器阵列。 描述了共焦技术以消除从物镜的焦平面外部散射的光。 这可以通过在镜头的焦点处将CCD的几个像素合并在一起，或者通过计算机中的图像处理技术来进行。