公开(公告)号：US20070289992A1

公开(公告)日：2007-12-20

申请号：US11542754

申请日：2006-10-02

申请人： David Nicol ,  Binh Nguyen ,  Peter Coassin ,  Walter Niles

发明人： David Nicol ,  Binh Nguyen ,  Peter Coassin ,  Walter Niles

IPC分类号： B65D47/18 ,  B67D5/08 ,  G01F11/00

CPC分类号： B01L3/0241 ,  B01L3/0275 ,  B01L2300/0838 ,  B01L2400/0439 ,  B01L2400/0487

摘要： Devices, systems, and methods for the precise dispensation of small volumes of liquids are presented. A fluid microdispenser comprises a liquid-filled tube, an actuator coupled to the tube, and a tip at one end of the tube. When the actuator applies an actuation pressure, a precise volume of liquid is ejected from the orifice of the tip. The orifice is manufactured to control the volume of liquid ejected by each actuation and, therefore, may have a diameter smaller than that of the liquid-filled tube. The invention comprises systems, methods, and devices so that the inner diameter of the liquid-filled tube is decreased (tapered) to that of the orifice so as to maximize transmission to the orifice of the pressure generated by the actuation stimulus. This enables the volume of liquid ejected by each stimulus to be reproducibly controlled by the amplitude of the actuation stimulus.

公开(公告)号：US20050054083A1

公开(公告)日：2005-03-10

申请号：US10837218

申请日：2004-04-30

申请人： Minh Vuong ,  Todd Bennett ,  Javier Flores ,  Brian Grot ,  Daniel Hale ,  Huy Nguyen ,  Walter Niles ,  Tuong Phan ,  Steve Rodems ,  Jeff Stack ,  Peter Coassin

发明人： Minh Vuong ,  Todd Bennett ,  Javier Flores ,  Brian Grot ,  Daniel Hale ,  Huy Nguyen ,  Walter Niles ,  Tuong Phan ,  Steve Rodems ,  Jeff Stack ,  Peter Coassin

IPC分类号： B23B27/04 ,  C12M1/34 ,  C12N20060101

CPC分类号： G01N35/1074 ,  G01N35/028

摘要： The invention is an automated multiple-purpose, integrated laboratory system comprising interchangeable modular elements for the construction and measurement of biological assays. The functions of the modular elements may include multiwell platform handling, chemical reagent or cell management, volumetric transfer of liquids for assay construction or for recovery of reaction products for analysis, incubation under controlled environmental conditions, measurement of spectrometric signals originating from the assays, processing and analysis of the resulting spectrometric data, and other functions. The modular elements are arranged around a number of robotic elements that deliver plates to different modular elements, transfer plates to groups of modules served by a different robotic element, or other actions necessary in plate handling. Liquid transfer to and from multiwell platforms, necessary for assay construction or for the initiation of physiological events in cells, is partitioned among different modules specialized for transferring nanoliter or smaller volume quantities of chemical concentrates, or microliter quantities of assay reagents, cells, media and other assay constituents. Applications of this invention include the quantitation and analysis of the expression of multiple genes in cells, measurement of multi-gene expression kinetics, analysis of activation or suppression of multiple signal transduction pathways, screening chemical compounds for modulatory effects on multi-gene expression or on signal transduction pathways or on other biochemical networks of cells, or other analytical biological or biochemical assays.

摘要翻译： 本发明是一种自动多功能综合实验室系统，包括用于构建和测量生物测定的可互换模块化元件。 模块化元件的功能可以包括多井平台处理，化学试剂或细胞管理，用于测定构建的液体的体积转移或用于回收用于分析的反应产物，在受控环境条件下孵育，来自测定的光谱信号的测量，处理 并对所得光谱数据进行分析等功能。 模块化元件围绕若干机器人元件布置，其将板递送到不同的模块元件，将板传递到由不同的机器人元件服务的模块组或板处理中所需的其他动作。 液体转移至多孔平台，用于测定结构或细胞中生理事件发生所必需的多孔平台，在专门用于转移纳升或更小体积量的化学浓缩物或微量的测定试剂，细胞，培养基和 其他检测成分。 本发明的应用包括细胞中多个基因的表达的定量和分析，多基因表达动力学的测定，多重信号转导途径的激活或抑制分析，筛选化合物对多基因表达的调节作用或 信号转导途径或细胞的其他生物化学网络或其他分析生物或生物化学测定。