SF3 promoter and methods of use
    6.
    发明授权
    SF3 promoter and methods of use 有权
    SF3启动子和使用方法

    公开(公告)号:US06452069B1

    公开(公告)日:2002-09-17

    申请号:US09524168

    申请日:2000-03-13

    IPC分类号: C12N1511

    摘要: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for pollen-preferred promoters isolated from a gene unit for SF3. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the pollen-preferred promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.

    摘要翻译: 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物是从用于SF3的基因单元分离的花粉优选启动子的新核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化为包含可操作地连接到本发明的花粉优选启动子之一的异源核苷酸序列,并从转化的植物细胞再生稳定转化的植物。

    Production of pathogen resistant plants
    8.
    发明授权
    Production of pathogen resistant plants 有权
    生产抗病虫害植物

    公开(公告)号:US06380460B1

    公开(公告)日:2002-04-30

    申请号:US09525174

    申请日:2000-03-14

    IPC分类号: A01H500

    摘要: Methods for increasing resistance in plants to pathogens by the expression of a hydrogen peroxide/reactive oxygen species producing enzyme or an oxalate degrading enzyme. The present invention relates to a method of producing a pathogen resistant hybrid plant by crossing the appropriate transgenic expressing a hydrogen peroxide/reactive oxygen species producing enzyme or an oxalate degrading enzyme with pathogen tolerant lines or inbreds obtained through conventional genetic manipulations, or by transformation of tolerant plants or plant tissues with a hydrogen peroxide/reactive oxygen species producing gene or by altering the expression of an endogenase hydrogen peroxide/reactive oxygen species producing gene. The synergistic effect of expression of a hydrogen peroxide/reactive oxygen species producing enzyme or an oxalate degrading enzyme in a tolerant background gives significant and unexpectedly high resistance to the pathogens.

    摘要翻译: 通过表达过氧化氢/活性氧产生酶或草酸降解酶来增加植物对病原体的抗性的方法。 本发明涉及通过将表达过氧化氢/活性氧生成酶或草酸降解酶的合适的转基因与通过常规遗传操作获得的病原体耐受品系或近交系交叉或通过转化获得的病原体抗性杂交植物的方法, 具有过氧化氢/活性氧产生基因的耐受植物或植物组织,或通过改变内切酶过氧化氢/活性氧产生基因的表达。 在耐受背景中,表达过氧化氢/活性氧产生酶或草酸降解酶的协同效应对病原体产生显着且出人意料的高抗性。