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公开(公告)号：US20140348813A1
公开(公告)日：2014-11-27
申请号：US14166619
申请日：2014-01-28
申请人： Donald W. Landry , James H. Woods , Roger K. Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Milan N. Stojanovich , John J. Tesmer , Remy L. Brim
发明人： Donald W. Landry , James H. Woods , Roger K. Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Milan N. Stojanovich , John J. Tesmer , Remy L. Brim
CPC分类号： A61K38/465 , A61K9/0019 , A61K31/505 , A61K45/06 , A61K47/12 , A61K47/18 , A61K47/22 , A61K47/542 , A61K47/545 , A61K47/60 , C12N9/18 , C12Q1/44 , C12Y301/01084 , G01N2333/918 , A61K2300/00
摘要： Provided are compositions comprising a cocaine esterase (CocE) and a compound that thermostabilizes the CocE. Also provided are methods of thermostabilizing a cocaine esterase. Additionally provided are methods of treating a mammal undergoing a cocaine-induced condition. Methods of determining whether a compound is a thermostabilizing agent for a protein are also provided. Uses of the above-described compositions for the treatment of a cocaine-induced condition is additionally provided. Additionally provided is an isolated nucleic acid encoding a CocE polypeptide having the substitutions L169K and G173Q, and the CocE polypeptide encoded by that nucleic acid, and pharmaceutical compositions thereof. Further provided is the use of that composition for the manufacture of a medicament for the treatment of a cocaine-induced condition and for the treatment of a cocaine-induced condition.
摘要翻译： 提供了包含可卡因酯酶（CocE）和使CocE稳定化合物的组合物。 还提供了热稳定可卡因酯酶的方法。 另外提供了治疗经历可卡因诱导病症的哺乳动物的方法。 还提供了确定化合物是否是蛋白质的热稳定剂的方法。 另外提供了上述用于治疗可卡因诱导病症的组合物的用途。 另外提供了编码具有取代L169K和G173Q的CocE多肽和由该核酸编码的CocE多肽的分离的核酸及其药物组合物。 还提供了该组合物用于制备用于治疗可卡因诱导的病症和用于治疗可卡因诱导病症的药物的用途。
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公开(公告)号：US20110142816A1
公开(公告)日：2011-06-16
申请号：US12667895
申请日：2008-07-10
申请人： Donald W. Landry , James H. Woods , Roger K. Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Milan N. Stojanovich , John J. Tesmer , Remy L. Brim
发明人： Donald W. Landry , James H. Woods , Roger K. Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Milan N. Stojanovich , John J. Tesmer , Remy L. Brim
CPC分类号： A61K38/465 , A61K9/0019 , A61K31/505 , A61K45/06 , A61K47/12 , A61K47/18 , A61K47/22 , A61K47/542 , A61K47/545 , A61K47/60 , C12N9/18 , C12Q1/44 , C12Y301/01084 , G01N2333/918 , A61K2300/00
摘要： Provided are compositions comprising a cocaine esterase (CocE) and a compound that thermostabilizes the CocE. Also provided are methods of thermostabilizing a cocaine esterase. Additionally provided are methods of treating a mammal under-going a cocaine-induced condition. Methods of determining whether a compound is a thermostabilizing agent for a protein are also provided. Uses of the above-described compositions for the treatment of a cocaine-induced condition is additionally provided. Additionally provided is an isolated nucleic acid encoding a CocE polypeptide having the substitutions L169K and G173Q, and the CocE polypeptide encoded by that nucleic acid, and pharmaceutical compositions thereof. Further provided is the use of that composition for the manufacture of a medicament for the treatment of a cocaine-induced condition and for the treatment of a cocaine-induced condition.
摘要翻译： 提供了包含可卡因酯酶（CocE）和使CocE稳定化合物的组合物。 还提供了热稳定可卡因酯酶的方法。 另外提供了治疗可卡因诱导病症的哺乳动物的方法。 还提供了确定化合物是否是蛋白质的热稳定剂的方法。 另外提供了上述用于治疗可卡因诱导病症的组合物的用途。 另外提供了编码具有取代L169K和G173Q的CocE多肽和由该核酸编码的CocE多肽的分离的核酸及其药物组合物。 还提供了该组合物用于制备用于治疗可卡因诱导的病症和用于治疗可卡因诱导病症的药物的用途。
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公开(公告)号：US08637009B2
公开(公告)日：2014-01-28
申请号：US12667895
申请日：2008-07-10
申请人： Donald W. Landry , James H. Woods , Roger K. Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Milan N. Stojanovich , John J. Tesmer , Remy L. Brim
发明人： Donald W. Landry , James H. Woods , Roger K. Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Milan N. Stojanovich , John J. Tesmer , Remy L. Brim
CPC分类号： A61K38/465 , A61K9/0019 , A61K31/505 , A61K45/06 , A61K47/12 , A61K47/18 , A61K47/22 , A61K47/542 , A61K47/545 , A61K47/60 , C12N9/18 , C12Q1/44 , C12Y301/01084 , G01N2333/918 , A61K2300/00
摘要： Provided are compositions comprising a cocaine esterase (CocE) and a compound that thermostabilizes the CocE. Also provided are methods of thermostabilizing a cocaine esterase. Additionally provided are methods of treating a mammal undergoing a cocaine-induced condition. Methods of determining whether a compound is a thermostabilizing agent for a protein are also provided. Uses of the above-described compositions for the treatment of a cocaine-induced condition is additionally provided. Additionally provided is an isolated nucleic acid encoding a CocE polypeptide having the substitutions L169K and G173Q, and the CocE polypeptide encoded by that nucleic acid, and pharmaceutical compositions thereof. Further provided is the use of that composition for the manufacture of a medicament for the treatment of a cocaine-induced condition and for the treatment of a cocaine-induced condition.
摘要翻译： 提供了包含可卡因酯酶（CocE）和使CocE稳定化合物的组合物。 还提供了热稳定可卡因酯酶的方法。 另外提供了治疗经历可卡因诱导病症的哺乳动物的方法。 还提供了确定化合物是否是蛋白质的热稳定剂的方法。 另外提供了上述用于治疗可卡因诱导病症的组合物的用途。 另外提供了编码具有取代L169K和G173Q的CocE多肽和由该核酸编码的CocE多肽的分离的核酸及其药物组合物。 还提供了该组合物用于制备用于治疗可卡因诱导的病症和用于治疗可卡因诱导病症的药物的用途。
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公开(公告)号：US09526786B2
公开(公告)日：2016-12-27
申请号：US14166619
申请日：2014-01-28
申请人： Donald W. Landry , James H. Woods , Roger K. Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Milan N. Stojanovic , John J. Tesmer , Remy L. Brim
发明人： Donald W. Landry , James H. Woods , Roger K. Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Milan N. Stojanovic , John J. Tesmer , Remy L. Brim
IPC分类号： A61K47/22 , C12N9/18 , C12N9/16 , A61K31/505 , A61K45/06 , A61K38/46 , A61K47/12 , A61K47/18 , C12Q1/44
CPC分类号： A61K38/465 , A61K9/0019 , A61K31/505 , A61K45/06 , A61K47/12 , A61K47/18 , A61K47/22 , A61K47/542 , A61K47/545 , A61K47/60 , C12N9/18 , C12Q1/44 , C12Y301/01084 , G01N2333/918 , A61K2300/00
摘要： Provided are compositions comprising a cocaine esterase (CocE) and a compound that thermostabilizes the CocE. Also provided are methods of thermostabilizing a cocaine esterase. Additionally provided are methods of treating a mammal undergoing a cocaine-induced condition. Methods of determining whether a compound is a thermostabilizing agent for a protein are also provided. Uses of the above-described compositions for the treatment of a cocaine-induced condition is additionally provided. Additionally provided is an isolated nucleic acid encoding a CocE polypeptide having the substitutions L169K and G173Q, and the CocE polypeptide encoded by that nucleic acid, and pharmaceutical compositions thereof. Further provided is the use of that composition for the manufacture of a medicament for the treatment of a cocaine-induced condition and for the treatment of a cocaine-induced condition.
摘要翻译： 提供了包含可卡因酯酶（CocE）和使CocE稳定化合物的组合物。 还提供了热稳定可卡因酯酶的方法。 另外提供了治疗经历可卡因诱导病症的哺乳动物的方法。 还提供了确定化合物是否是蛋白质的热稳定剂的方法。 另外提供了上述用于治疗可卡因诱导病症的组合物的用途。 另外提供了编码具有取代L169K和G173Q的CocE多肽和由该核酸编码的CocE多肽的分离的核酸及其药物组合物。 还提供了该组合物用于制备用于治疗可卡因诱导的病症和用于治疗可卡因诱导病症的药物的用途。
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公开(公告)号：US20170106056A1
公开(公告)日：2017-04-20
申请号：US15390514
申请日：2016-12-25
申请人： Donald W. Landry , James H. Woods , Roger K. Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Milan N. Stojanovic , John J. Tesmer , Remy L. Brim
发明人： Donald W. Landry , James H. Woods , Roger K. Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Milan N. Stojanovic , John J. Tesmer , Remy L. Brim
CPC分类号： A61K38/465 , A61K9/0019 , A61K31/505 , A61K45/06 , A61K47/12 , A61K47/18 , A61K47/22 , A61K47/542 , A61K47/545 , A61K47/60 , C12N9/18 , C12Q1/44 , C12Y301/01084 , G01N2333/918 , A61K2300/00
摘要： Provided are compositions comprising a cocaine esterase (CocE) and a compound that thermostabilizes the CocE. Also provided are methods of thermostabilizing a cocaine esterase. Additionally provided are methods of treating a mammal undergoing a cocaine-induced condition. Methods of determining whether a compound is a thermostabilizing agent for a protein are also provided. Uses of the above-described compositions for the treatment of a cocaine-induced condition is additionally provided. Additionally provided is an isolated nucleic acid encoding a CocE polypeptide having the substitutions L169K and G173Q, and the CocE polypeptide encoded by that nucleic acid, and pharmaceutical compositions thereof. Further provided is the use of that composition for the manufacture of a medicament for the treatment of a cocaine-induced condition and for the treatment of a cocaine-induced condition.
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公开(公告)号：US08318156B2
公开(公告)日：2012-11-27
申请号：US12373510
申请日：2007-07-10
申请人： Donald W Landry , Joanne MacDonald , Shi-Xian Deng , Chang-Guo Zhan , Daquan Gao , James H. Woods , Roger K. Sunahara , Diwahar L. Narasimhan , Victor Yang , Mei-Chuan Holden Ko , John J. Tesmer , Tien-Yi Lee , Young Min Kwon
发明人： Donald W Landry , Joanne MacDonald , Shi-Xian Deng , Chang-Guo Zhan , Daquan Gao , James H. Woods , Roger K. Sunahara , Diwahar L. Narasimhan , Victor Yang , Mei-Chuan Holden Ko , John J. Tesmer , Tien-Yi Lee , Young Min Kwon
CPC分类号： C12N9/18 , A61K38/00 , A61K38/465 , C12Y301/01084
摘要： Embodiments of the invention disclosed herein generally relate to anti-cocaine therapeutics. Specifically, some embodiments of the invention relate to highly efficient, thermostable, and long-lasting cocaine esterase (CocE) mutants that can protect against the toxic and reinforcing effects of cocaine in subjects. Provided herein are mutant CocE polypeptides displaying thermostable esterase activity. Also provided are methods of treating cocaine-induced conditions in a subject in need via administration of mutant CocE as well as methods for high-throughput screening of candidate esterase polypeptides.
摘要翻译： 本文公开的本发明的实施方案通常涉及抗可卡因治疗剂。 具体地，本发明的一些实施方案涉及可以防止可卡因在受试者中的有毒和增强作用的高效，耐热和持久的可卡因酯酶（CocE）突变体。 本文提供显示耐热酯酶活性的突变型CocE多肽。 还提供了通过施用突变体CocE处理需要的受试者中可卡因诱导的病症的方法以及候选酯酶多肽的高通量筛选方法。
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公开(公告)号：US20160160195A1
公开(公告)日：2016-06-09
申请号：US14954813
申请日：2015-11-30
申请人： Donald Landry , Chang-Guo Zhan , James H. Woods , Roger Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Victor Yang , Mei-Chuan Holden Ko , Shi-Xian Deng , John J. Tesmer , Tien-Yi Lee , Young Min Kwon , Daquan Gao
发明人： Donald Landry , Chang-Guo Zhan , James H. Woods , Roger Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Victor Yang , Mei-Chuan Holden Ko , Shi-Xian Deng , John J. Tesmer , Tien-Yi Lee , Young Min Kwon , Daquan Gao
CPC分类号： C12N9/18 , A61K38/00 , A61K38/465 , C12Y301/01084
摘要： Embodiments of the invention disclosed herein generally relate to anti-cocaine therapeutics. Specifically, some embodiments of the invention relate to highly efficient, thermostable, and long-lasting cocaine esterase (CocE) mutants that can protect against the toxic and reinforcing effects of cocaine in subjects. Provided herein are mutant CocE polypeptides displaying thermostable esterase activity. Also provided are methods of treating cocaine-induced conditions in a subject in need via administration of mutant CocE as well as methods for high-throughput screening of candidate esterase polypeptides.
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公开(公告)号：US20100034799A1
公开(公告)日：2010-02-11
申请号：US12373510
申请日：2007-07-10
申请人： Donald Landry , Chang-Guo Zhan , James H. Woods , Roger Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Victor Yang , Mei-Chuan Holden Ko , Shi-Xian Deng , John J. Tesmer , Tien-Yi Lee , Young Min Kwon , Daquan Gao
发明人： Donald Landry , Chang-Guo Zhan , James H. Woods , Roger Sunahara , Diwahar L. Narasimhan , Joanne MacDonald , Victor Yang , Mei-Chuan Holden Ko , Shi-Xian Deng , John J. Tesmer , Tien-Yi Lee , Young Min Kwon , Daquan Gao
CPC分类号： C12N9/18 , A61K38/00 , A61K38/465 , C12Y301/01084
摘要： Embodiments of the invention disclosed herein generally relate to anti-cocaine therapeutics. Specifically, some embodiments of the invention relate to highly efficient, thermostable, and long-lasting cocaine esterase (CocE) mutants that can protect against the toxic and reinforcing effects of cocaine in subjects. Provided herein are mutant CocE polypeptides displaying thermostable esterase activity. Also provided are methods of treating cocaine-induced conditions in a subject in need via administration of mutant CocE as well as methods for high-throughput screening of candidate esterase polypeptides.
摘要翻译： 本文公开的本发明的实施方案通常涉及抗可卡因治疗剂。 具体地，本发明的一些实施方案涉及可以防止可卡因在受试者中的有毒和增强作用的高效，耐热和持久的可卡因酯酶（CocE）突变体。 本文提供显示耐热酯酶活性的突变型CocE多肽。 还提供了通过施用突变体CocE处理需要的受试者中可卡因诱导的病症的方法以及候选酯酶多肽的高通量筛选方法。
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公开(公告)号：US08119782B2
公开(公告)日：2012-02-21
申请号：US12319835
申请日：2009-01-12
IPC分类号： C07H21/04
CPC分类号： C12Q1/6823 , C12Q2563/107 , C12Q2521/337
摘要： The development of the first solution-phase molecular assembly comprising over 100 molecular logic gates, which more than quadruples the complexity performed by any previous system. “MAYA-II” is a second generation molecular automaton capable of playing a complete game of tic-tac-toe against a human opponent, and encompasses 76 permissible game plays. MAYA-II is more user-friendly than MAYA-I, as it signals both players move in a two-color output system and imposes no constraints on the position of the human player's first move. MAYA-II is constructed from three classes of stem-loop controlled deoxyribozyme-based logic gates that are allosterically modulated by input oligonucleotides to produce fluorescent output signals.
摘要翻译： 第一个溶液相分子组装的开发包括超过100个分子逻辑门，其比任何先前系统执行的复杂性高四倍以上。 “MAYA-II”是第二代分子自动机，能够针对人类对手进行完整的Tic-tac-toe游戏，包括76个允许的游戏。 MAYA-II比MAYA-I更加用户友好，因为它表示两名玩家在双色输出系统中移动，并且对玩家第一举的位置没有约束。 MAYA-II由三类基于茎环控制的脱氧核酶的逻辑门构成，其由输入寡核苷酸变构调制以产生荧光输出信号。
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公开(公告)号：US20090275027A1
公开(公告)日：2009-11-05
申请号：US12319835
申请日：2009-01-12
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q2563/107 , C12Q2521/337
摘要： Compositions and methods for optically detecting the presence of a plurality of oligonucleotides in a sample, wherein each oligonucleotide to be detected comprises consecutive nucleotides having a sequence different than the remaining oligonucleotides of the plurality are provided.
摘要翻译： 用于光学检测样品中多个寡核苷酸的存在的组合物和方法，其中待检测的每个寡核苷酸包括具有与多个残基的寡核苷酸不同的序列的连续核苷酸。
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