公开(公告)号：US20050208678A1

公开(公告)日：2005-09-22

申请号：US10490716

申请日：2002-09-27

申请人： Dong Xie ,  John Erickson ,  Paul Grulich

发明人： Dong Xie ,  John Erickson ,  Paul Grulich

IPC分类号： A61K45/00 ,  A61P31/18 ,  C07K7/00 ,  C07K14/47 ,  G01N27/447 ,  G01N33/15 ,  G01N33/48 ,  G01N33/50 ,  G01N33/561 ,  G01N33/569 ,  G01N33/68 ,  G06F19/00

CPC分类号： G01N33/56988 ,  G01N33/6803 ,  G01N2333/162 ,  G01N2500/02

摘要： Methods of identifying a fusion inhibitor and inhibitors of gp41-mediated membrane fusion are disclosed. The methods comprise, for example, providing a first helical polypeptide comprising a sequence of IQN17 (SEQ ID NO: 1); providing a second helical polypeptide of 34 or less than 34 amino acids comprising the amino acid sequence W-X1-X2-W-X3-X4-X5-I, wherein X1, X2, X3, X4, and X5 are each independently chosen from any amino acid except proline; measuring, by capillary zone electrophoresis, the degree of complex formation between these peptides; and comparing the measured degree of complex formation to the degree in the presence of a test composition.

摘要翻译： 公开了鉴定融合抑制剂和gp41介导的膜融合抑制剂的方法。 所述方法包括例如提供包含IQN17（SEQ ID NO：1）序列的第一螺旋多肽; 提供包含氨基酸序列W-X1-X2-W-X3-X4-X5-I的34个或小于34个氨基酸的第二螺旋多肽，其中X1，X2，X3，X4和X5各自独立地选自 除脯氨酸以外的任何氨基酸; 通过毛细管区带电泳测量这些肽之间的复合物形成程度; 并将测量的复合物形成程度与存在测试组合物的程度进行比较。