PLANT PROMOTOR AND 3' UTR FOR TRANSGENE EXPRESSION
    3.
    发明申请
    PLANT PROMOTOR AND 3' UTR FOR TRANSGENE EXPRESSION 审中-公开
    植物促进剂和3'UTR用于转化表达

    公开(公告)号:US20170051298A1

    公开(公告)日:2017-02-23

    申请号:US15233328

    申请日:2016-08-10

    Abstract: This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a promoter and/or a 3′UTR from Zea mays Zrp2 gene. Some embodiments relate to a promoter from a Zea mays Zrp2 gene that is operably linked to a Zea mays Ubiquitin 1 intron and functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR from a Zea mays Zrp2 gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

    Abstract translation: 本公开涉及用于促进植物或植物细胞中的核苷酸序列的转录和翻译的组合物和方法,其使用来自玉蜀黍ZrP2基因的启动子和/或3'UTR。 一些实施方案涉及可操作地连接到玉米泛素1内含子的Zea mays Zrp2基因的启动子,并在植物中起作用以促进可操作地连接的核苷酸序列的转录。 其他实施方案涉及在植物中起作用以终止可操作地连接的核苷酸序列的转录的Zea mays Zrp2基因的3'UTR。

    USE OF A MAIZE UNTRANSLATED REGION FOR TRANSGENE EXPRESSION IN PLANTS

    公开(公告)号:US20200277621A1

    公开(公告)日:2020-09-03

    申请号:US16648109

    申请日:2018-09-07

    Abstract: Provided are methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 3′ untranslated regions of Zea mays chlorophyll a/b binding protein gene and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional or gene targeting procedures, resulting in enhanced expression of the selected coding region. In some embodiments, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

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