公开(公告)号：US20150064790A1

公开(公告)日：2015-03-05

申请号：US14538609

申请日：2014-11-11

申请人： Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

发明人： Manju Gupta ,  Asha M. Palta ,  Stephen Novak ,  Fyodor Urnov ,  Sunita Gopalan

IPC分类号： C12N15/82

CPC分类号： C12N15/8213 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/1092 ,  C12N15/8243 ,  C12N15/8274

摘要： The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

摘要翻译： 本公开涉及靶向植物中5-烯醇丙酮酸莽草酸-3-磷酸合酶（EPSPS）基因的工程化锌指蛋白，以及使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。 特别地，本公开涉及用于靶向切割和改变EPSPS基因的锌指核酸酶。

公开(公告)号：US08889390B2

公开(公告)日：2014-11-18

申请号：US13766380

申请日：2013-02-13

申请人： Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

发明人： Manju Gupta ,  Asha M. Palta ,  Stephen Novak ,  Fyodor Urnov ,  Sunita Gopalan

IPC分类号： C12N9/00 ,  C12N15/82 ,  C12N9/10

CPC分类号： C12N15/8213 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/1092 ,  C12N15/8243 ,  C12N15/8274

摘要： The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

摘要翻译： 本公开涉及靶向植物中5-烯醇丙酮酸莽草酸-3-磷酸合酶（EPSPS）基因的工程化锌指蛋白，以及使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。 特别地，本公开涉及用于靶向切割和改变EPSPS基因的锌指核酸酶。

公开(公告)号：US10344289B2

公开(公告)日：2019-07-09

申请号：US14538609

申请日：2014-11-11

申请人： Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

发明人： Manju Gupta ,  Asha M. Palta ,  Stephen Novak ,  Fyodor Urnov ,  Sunita Gopalan

IPC分类号： C12N15/66 ,  C12N15/62 ,  C12N15/82 ,  C12N9/10

摘要： The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

公开(公告)号：US20130145503A1

公开(公告)日：2013-06-06

申请号：US13766380

申请日：2013-02-13

申请人： c/o Dow AgroSciences LLC ,  c/o Sangamo BioSciences, Inc.

发明人： Manju Gupta ,  Asha M. Palta ,  Stephen Novak ,  Fyodor Urnov ,  Sunita Gopalan

IPC分类号： C12N15/82

CPC分类号： C12N15/8213 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/1092 ,  C12N15/8243 ,  C12N15/8274

摘要： The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.