公开(公告)号：US10788450B2

公开(公告)日：2020-09-29

申请号：US15546671

申请日：2016-01-08

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Yitao Long ,  Chan Cao ,  Yongxu Hu

IPC: C12Q1/6869 ,  G01N33/487 ,  G01N27/447

Abstract: A preparation method for an aerolysin nanopore in this disclosure comprises the following steps: (1) pretreatment of an aerolysin; (2) preparation of a lipid bilayer membrane by pulling process; (3) forming of the aerolysin nanopore: the aerolysin nanopore is obtained at a current of 50±5 pA. The aerolysin nanopore prepared in the invention is structurally stable and has a high resolution with the whole internal cavity carried with a positive charge, can be used for detection without modification and is easily operated. Further, the aerolysin nanopore can be applied in DNA sequencing, DNA damage and Micro-RNA detection.