公开(公告)号：US11001795B2

公开(公告)日：2021-05-11

申请号：US15819966

申请日：2017-11-21

Applicant: EMULATE, Inc.

Inventor： S. Jordan Kerns ,  Jenifer Obrigewitch ,  Michael Salmon ,  Magdalena Kasendra ,  Benjamin Richards Umiker

IPC: C12M3/06 ,  G01N33/50 ,  B01L3/00 ,  C12N5/077 ,  C12N5/071 ,  C07K16/28 ,  C12M1/12 ,  C12N5/0783

Abstract: An in vitro microfluidic gut-on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and gastrointestinal epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal tissue, e.g., Crohn's disease, colitis and other inflammatory gastrointestinal disorders. These multicellular, layered microfluidic gut-on-chip further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal deuodejeum, small intestinal ileium, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

公开(公告)号：US20220081663A1

公开(公告)日：2022-03-17

申请号：US17527422

申请日：2021-11-16

Applicant: EMULATE, INC.

Inventor： S. Jordan Kerns ,  Jenifer Obrigewitch ,  Micheal Salmon ,  Benjamin Richards Umiker

IPC: C12M3/06 ,  G01N33/50 ,  B01L3/00 ,  C12N5/077 ,  C12N5/071 ,  C07K16/28 ,  C12M1/12 ,  C12N5/0783

Abstract: An in vitro microfluidic gut-on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and gastrointestinal epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal tissue, e.g., Crohn's disease, colitis and other inflammatory gastrointestinal disorders. These multicellular, layered microfluidic gut-on-chip further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal deuodejeum, small intestinal ileium, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

公开(公告)号：US20180230417A1

公开(公告)日：2018-08-16

申请号：US15820011

申请日：2017-11-21

Applicant: EMULATE, Inc.

Inventor： S. Jordan Kerns ,  Jenifer Obrigewitch ,  Michael Salmon ,  Benjamin Richards Umiker

IPC: C12M3/06 ,  C12M1/12 ,  C07K16/28 ,  C12N5/0783 ,  G01N33/50

CPC classification number: C12M23/16 ,  B01L3/502753 ,  B01L2300/0681 ,  C07K16/2809 ,  C07K16/2818 ,  C12M25/02 ,  C12N5/0636 ,  C12N5/0652 ,  C12N5/0679 ,  C12N5/0697 ,  C12N2501/999 ,  C12N2502/13 ,  C12N2502/23 ,  C12N2502/28 ,  C12N2503/04 ,  C12N2533/90 ,  C12N2533/92 ,  G01N33/505 ,  G01N33/5088

Abstract: An in vitro microfluidic gut-on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and gastrointestinal epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal tissue, e.g., Crohn's disease, colitis and other inflammatory gastrointestinal disorders. These multicellular, layered microfluidic gut-on-chip further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal deuodejeum, small intestinal ileium, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

公开(公告)号：US11230688B2

公开(公告)日：2022-01-25

申请号：US15820011

申请日：2017-11-21

Applicant: EMULATE, Inc.

Inventor： S. Jordan Kerns ,  Jenifer Obrigewitch ,  Michael Salmon ,  Benjamin Richards Umiker

IPC: C12M3/06 ,  G01N33/50 ,  B01L3/00 ,  C12N5/077 ,  C12N5/071 ,  C07K16/28 ,  C12M1/12 ,  C12N5/0783

Abstract: An in vitro microfluidic gut-on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and gastrointestinal epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal tissue, e.g., Crohn's disease, colitis and other inflammatory gastrointestinal disorders. These multicellular, layered microfluidic gut-on-chip further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal deuodejeum, small intestinal ileium, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

公开(公告)号：US20180224432A1

公开(公告)日：2018-08-09

申请号：US15819966

申请日：2017-11-21

Applicant: EMULATE, Inc.

Inventor： S. Jordan Kerns ,  Jenifer Obrigewitch ,  Michael Salmon ,  Magdalena Kasendra ,  Benjamin Richards Umiker

IPC: G01N33/50 ,  B01L3/00 ,  C12N5/077 ,  C12N5/071

CPC classification number: C12M23/16 ,  B01L3/502753 ,  B01L2300/0681 ,  C07K16/2809 ,  C07K16/2818 ,  C12M25/02 ,  C12N5/0636 ,  C12N5/0652 ,  C12N5/0679 ,  C12N5/0697 ,  C12N2501/999 ,  C12N2502/13 ,  C12N2502/23 ,  C12N2502/28 ,  C12N2503/04 ,  C12N2533/90 ,  C12N2533/92 ,  G01N33/505 ,  G01N33/5088

Abstract: An in vitro microfluidic gut-on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and gastrointestinal epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal tissue, e.g., Crohn's disease, colitis and other inflammatory gastrointestinal disorders. These multicellular, layered microfluidic gut-on-chip further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal deuodejeum, small intestinal ileium, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.