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公开(公告)号：US20190002491A1
公开(公告)日：2019-01-03
申请号：US16127950
申请日：2018-09-11
发明人： Zaiguo Li , Praveen Pande
摘要： Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.
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公开(公告)号：US20160311846A1
公开(公告)日：2016-10-27
申请号：US15205438
申请日：2016-07-08
发明人： Zaiguo Li , Praveen Pande
摘要： Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.
摘要翻译： 一种方法实施方案包括使荧光染料化合物的反应性基团Z与靶分子接触，使得反应性基团Z与靶分子反应以在基团和靶分子之间形成共价键。 另一种方法实施方案包括使进一步包含结合对的第一个成员的荧光染料化合物与包含结合对的第二个成员的靶分子接触。 还提供了用荧光染料化合物标记的靶分子。
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