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1.发明申请公开(公告)号：US20080024861A1
公开(公告)日：2008-01-31
申请号：US11828617
申请日：2007-07-26
IPC分类号： G02B21/26
摘要： The invention is directed to an arrangement for regulating the temperature of the sample space of a microscope, preferably for observing cells (live cell imaging) with an objective turret carrying objectives, a sample holder serving to receive a sample, a heating or cooling insert with an incubator hood for managing the temperature of the sample holder, and an objective heating element or objective cooling element. According to the invention, at least one structural component part which blocks the heating flow or cooling flow is provided between the objective heating element or objective cooling element and the objective turret.
摘要翻译： 本发明涉及一种用于调节显微镜样品空间温度的装置，优选用于观察携带物镜的目标转盘（活细胞成像），用于接收样品的样品架，加热或冷却插入物 用于管理样品保持器的温度的孵化器罩，以及客观加热元件或目标冷却元件。 根据本发明，在目标加热元件或目标冷却元件和物镜转台之间设置有阻挡加热流或冷却流的至少一个结构部件。
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2.发明授权公开(公告)号：US07502165B2
公开(公告)日：2009-03-10
申请号：US11828617
申请日：2007-07-26
IPC分类号： G02B21/26
摘要： The invention is directed to an arrangement for regulating the temperature of the sample space of a microscope, preferably for observing cells (live cell imaging) with an objective turret carrying objectives, a sample holder serving to receive a sample, a heating or cooling insert with an incubator hood for managing the temperature of the sample holder, and an objective heating element or objective cooling element. According to the invention, at least one structural component part which blocks the heating flow or cooling flow is provided between the objective heating element or objective cooling element and the objective turret.
摘要翻译： 本发明涉及一种用于调节显微镜样品空间温度的装置，优选用于观察携带物镜的目标转盘（活细胞成像），用于接收样品的样品架，加热或冷却插入物 用于管理样品保持器的温度的孵化器罩，以及客观加热元件或目标冷却元件。 根据本发明，在目标加热元件或目标冷却元件和物镜转台之间设置有阻挡加热流或冷却流的至少一个结构部件。
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3.发明申请公开(公告)号：US20060186311A1
公开(公告)日：2006-08-24
申请号：US10533067
申请日：2003-10-28
申请人： Bernhard Backs , Joerg Schaffer , Lutz Schaefer , Martin Voelcker
发明人： Bernhard Backs , Joerg Schaffer , Lutz Schaefer , Martin Voelcker
IPC分类号： G02B27/40
CPC分类号： G02B21/0004 , G02B21/361
摘要： The invention is directed to a method for improving the depth discrimination in optically imaging systems. It is applicable in particular in light microscopy for improving image quality when examining three-dimensionally extending objects. It is applicable in the method of structured illumination as described in WO 97/6509. For this purpose, influences due to variations in the brightness of the light source, positioning of the imaged periodic structure and bleaching of the object in fluorescence illumination are determined and taken into account in the calculation of the object structure.
摘要翻译： 本发明涉及一种用于改善光学成像系统中的深度辨别的方法。 特别适用于光学显微镜，用于在检查三维扩展物体时提高图像质量。 它适用于如WO 97/6509中所述的结构照明方法。 为此，在物体结构的计算中确定并考虑了由于光源的亮度变化，成像周期性结构的定位和物体在荧光照明中的漂白而引起的影响。
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公开(公告)号：US09405111B2
公开(公告)日：2016-08-02
申请号：US14008999
申请日：2012-02-27
申请人： Joerg Schaffer , Peter Schnuell
发明人： Joerg Schaffer , Peter Schnuell
CPC分类号： G02B21/0092 , G02B7/006 , G02B21/14 , G02B21/34
摘要： The invention relates to an optical assembly that can be interposed into the observation beam path of a microscope, comprising a first mount. In the first mount, a stack of optical elements for a polarization optical, differential interference contrast method, is arranged to facilitate a first observation method. The stack comprises, inter alia, a polarizer, polarization-optical shearing elements, and an analyzer. The analyzer is arranged in the stack with regard to its polarization direction in a predetermined orientation relative to the polarization direction of the polarizer. The stack of optical elements in the first mount is arranged such as to be interchangeable. Further, the assembly comprises at least one additional mount for receiving optical elements for at least one additional observation method.
摘要翻译： 本发明涉及一种可以插入显微镜的观察光束路径中的光学组件，包括第一安装件。 在第一安装件中，布置了用于偏振光学，差分干涉对比方法的一叠光学元件，以便于第一观察方法。 该叠层尤其包括偏振器，偏振光学剪切元件和分析器。 相对于其偏振方向，分析器相对于偏振器的偏振方向以预定方向布置在堆叠中。 第一安装件中的光学元件堆叠布置成可互换。 此外，组件包括用于接收用于至少一个附加观察方法的光学元件的至少一个附加安装件。
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公开(公告)号：US20140153088A1
公开(公告)日：2014-06-05
申请号：US14008999
申请日：2012-02-27
申请人： Joerg Schaffer , Peter Schnuell
发明人： Joerg Schaffer , Peter Schnuell
IPC分类号： G02B21/00
CPC分类号： G02B21/0092 , G02B7/006 , G02B21/14 , G02B21/34
摘要： The invention relates to an optical assembly that can be interposed into the observation beam path of a microscope, comprising a first mount. In the first mount, a stack of optical elements for a polarization optical, differential interference contrast method, is arranged to facilitate a first observation method. The stack comprises, inter alia, a polarizer, polarization-optical shearing elements, and an analyzer. The analyzer is arranged in the stack with regard to its polarization direction in a predetermined orientation relative to the polarization direction of the polarizer. The stack of optical elements in the first mount is arranged such as to be interchangeable. Further, the assembly comprises at least one additional mount for receiving optical elements for at least one additional observation method.
摘要翻译： 本发明涉及一种可以插入显微镜的观察光束路径中的光学组件，包括第一安装件。 在第一安装件中，布置了用于偏振光学，差分干涉对比方法的一叠光学元件，以便于第一观察方法。 该叠层尤其包括偏振器，偏振光学剪切元件和分析器。 相对于其偏振方向，分析器相对于偏振器的偏振方向以预定方向布置在堆叠中。 第一安装件中的光学元件堆叠布置成可互换。 此外，组件包括用于接收用于至少一个附加观察方法的光学元件的至少一个附加安装件。
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6.发明授权公开(公告)号：US07335866B2
公开(公告)日：2008-02-26
申请号：US10533067
申请日：2003-10-28
申请人： Bernhard Backs , Joerg Schaffer , Lutz Schaefer , Martin Voelcker
发明人： Bernhard Backs , Joerg Schaffer , Lutz Schaefer , Martin Voelcker
CPC分类号： G02B21/0004 , G02B21/361
摘要： The invention is directed to a method for improving the depth discrimination in optically imaging systems. It is applicable in particular in light microscopy for improving image quality when examining three-dimensionally extending objects. It is applicable in the method of structured illumination as described in WO 97/6509. For this purpose, influences due to variations in the brightness of the light source, positioning of the imaged periodic structure and bleaching of the object in fluorescence illumination are determined and taken into account in the calculation of the object structure.
摘要翻译： 本发明涉及一种用于改善光学成像系统中的深度辨别的方法。 特别适用于光学显微镜，用于在检查三维扩展物体时提高图像质量。 它适用于如WO 97/6509中所述的结构照明方法。 为此，在物体结构的计算中确定并考虑了由于光源的亮度变化，成像周期性结构的定位和物体在荧光照明中的漂白而引起的影响。
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7.发明授权公开(公告)号：US09341833B2
公开(公告)日：2016-05-17
申请号：US13991123
申请日：2011-09-13
申请人： Joerg Schaffer , Tim Plohnke
发明人： Joerg Schaffer , Tim Plohnke
CPC分类号： G02B21/06 , G02B21/0032 , G02B21/004 , G02B21/0076
摘要： A device for increasing the depth discrimination of optical imaging systems includes elements for structuring illumination light in an illumination beam of the optical imaging system by application of a grating-like pattern in a conjugate object plane of the optical imaging system. The device further elements for varying the position of the grating-like patterns along an optical axis of the illuminating beam, and elements that vary the position of an image of the grating-like pattern on a specimen in an object plane. The invention may include a grating changer connected to an electronic control system or to a control program of the optical imaging system for automatically changing the grating-like pattern to at least one further grating-like pattern and/or for removing the grating like pattern from the illuminating beam path.
摘要翻译： 用于增加光学成像系统的深度鉴别的装置包括用于通过在光学成像系统的共轭物体平面中施加格子状图案来在光学成像系统的照明光束中构造照明光的元件。 所述装置还具有用于沿着照明光束的光轴改变格栅状图案的位置的元件，以及改变对象平面中的样本上的格子状图案的图像的位置的元件。 本发明可以包括连接到电子控制系统或光学成像系统的控制程序的光栅更换器，用于将光栅样图案自动地改变为至少一个另外的格栅状图案和/或用于从 照明光束路径。
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8.发明申请公开(公告)号：US20130250407A1
公开(公告)日：2013-09-26
申请号：US13991123
申请日：2011-09-13
申请人： Joerg Schaffer , Tim Plohnke
发明人： Joerg Schaffer , Tim Plohnke
IPC分类号： G02B21/06
CPC分类号： G02B21/06 , G02B21/0032 , G02B21/004 , G02B21/0076
摘要： The invention relates to a device for increasing the depth discrimination of optical imaging systems. Such a device comprises means for structuring illumination light in an illumination beam of the optical imaging system by means of a grating-like pattern in a conjugated object plane of the optical imaging system. The device further comprises means for varying the position of the grating-like patterns along an optical axis of the illuminating beam, and means for varying the position of an image of the grating-like pattern on a specimen in an object plane.Such a device is characterized by a grating changer connected to an electronic control system or to a control program of the optical imaging system for automatically changing the grating-like pattern to at least one further grating-like pattern and/or for removing the grating-like pattern from the illuminating beam path.
摘要翻译： 本发明涉及用于增加光学成像系统的深度辨别的装置。 这种装置包括用于通过光学成像系统的共轭物体平面中的格子状图案在光学成像系统的照明光束中构造照明光的装置。 该装置还包括用于沿着照明光束的光轴改变格子状图案的位置的装置，以及用于改变物体平面中的样本上的格子状图案的图像的位置的装置。 这种装置的特征在于连接到电子控制系统或光学成像系统的控制程序的光栅更换器，用于将光栅样图案自动地改变为至少一个另外的格栅状图案和/或用于去除光栅 - 来自照明光束路径的类似图案。
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公开(公告)号：US07538941B2
公开(公告)日：2009-05-26
申请号：US11548133
申请日：2006-10-10
申请人： Joerg Schaffer
发明人： Joerg Schaffer
CPC分类号： G02B21/16
摘要： The invention is directed to a method and a device for imaging structures either in the focal plane of the objective of a microscope or in the pupil of the objective or in the respective conjugate planes. For this purpose, the optical data of the imaging systems and the illumination wavelengths or fluorescence wavelengths are taken into account when determining the optimal position of the structures.
摘要翻译： 本发明涉及用于在显微镜的物镜的焦平面或物镜的瞳孔或各个共轭平面中成像结构的方法和装置。 为此，当确定结构的最佳位置时，考虑成像系统的光学数据和照明波长或荧光波长。
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