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公开(公告)号：US10125360B2
公开(公告)日：2018-11-13
申请号：US15411628
申请日：2017-01-20
Inventor： Kihwan Kwon
IPC: C12N13/00 , C12N15/113
Abstract: The present invention relates to a method for promoting the generation and secretion of extracellular vesicles from cells by using extracorporeal shockwave. More precisely, the inventors confirmed that the generation and secretion of extracellular vesicles from vascular endothelial cells were promoted by extracorporeal shockwave and further confirmed that siRNA can be transferred into cells without any help of a carrier. The inventors continued to find out that angiogenesis could be inhibited by introducing siRNA to an animal tumor model and succeeded to measure the size and amount of extracellular vesicles secreted by extracorporeal shockwave. Therefore, the present invention can be used in various fields since it can safely promote the generation and secretion of extracellular vesicles from cells.
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2.发明申请公开(公告)号：US20170204396A1
公开(公告)日：2017-07-20
申请号：US15411628
申请日：2017-01-20
Inventor： Kihwan Kwon
IPC: C12N13/00 , C12N15/113
CPC classification number: C12N13/00 , C12N15/113 , C12N15/1136 , C12N15/1138 , C12N2310/14 , C12N2310/3517 , C12N2320/32
Abstract: The present invention relates to a method for promoting the generation and secretion of extracellular vesicles from cells by using extracorporeal shockwave. More precisely, the inventors confirmed that the generation and secretion of extracellular vesicles from vascular endothelial cells were promoted by extracorporeal shockwave and further confirmed that siRNA can be transferred into cells without any help of a carrier. The inventors continued to find out that angiogenesis could be inhibited by introducing siRNA to an animal tumor model and succeeded to measure the size and amount of extracellular vesicles secreted by extracorporeal shockwave. Therefore, the present invention can be used in various fields since it can safely promote the generation and secretion of extracellular vesicles from cells.
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3.发明申请公开(公告)号：US20180344643A1
公开(公告)日：2018-12-06
申请号：US15995638
申请日：2018-06-01
Inventor： Kihwan Kwon , Jihwa Chung , Kyounghwa Kim
Abstract: The present disclosure relates to a method of delivering target materials into extracellular vesicles including exposing the target materials and the extracellular vesicles to extracorporeal shockwaves, a method of preparing target material-introduced extracellular vesicles, extracellular vesicles prepared by the method, drug delivery vehicles including extracellular vesicles, and a method of delivering target materials into cells. The present disclosure exposes extracellular vesicles derived from natural organisms such as animal cells, plant cells, and microorganisms including bacteria and eukaryotic bacteria as well as artificially produced extracellular vesicles to extracorporeal shockwaves extracellularly. Thus, the high-level energy extracorporeal shockwaves can be used to deliver the target material into the extracellular vesicle efficiently. When treating with extracorporeal shockwaves, the ability of target material-introduced extracellular vesicles to incorporate into target cells also increases. According to the method of the present disclosure, the target materials can be delivered into cells with high efficiency, being utilized in various fields.
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