公开(公告)号：US12123012B2

公开(公告)日：2024-10-22

申请号：US17104552

申请日：2020-11-25

申请人： LIGANDAL, INC.

发明人： Andre Ronald Watson ,  Christian Foster

IPC分类号： A61K48/00 ,  A61K9/00 ,  A61K9/51 ,  A61K31/40 ,  A61K31/7105 ,  A61K47/34 ,  A61K47/42 ,  A61K47/54 ,  A61K47/64 ,  A61K47/68 ,  A61P3/10 ,  A61P35/00 ,  A61P37/00 ,  B82Y5/00 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12N15/87 ,  C12N9/22

CPC分类号： C12N15/85 ,  A61K9/0019 ,  A61K9/0085 ,  A61K9/5146 ,  A61K31/40 ,  A61K31/7105 ,  A61K47/34 ,  A61K47/42 ,  A61K47/549 ,  A61K47/64 ,  A61K47/645 ,  A61K47/6455 ,  A61K47/6807 ,  A61K48/0016 ,  A61K48/0041 ,  A61K48/0075 ,  A61P3/10 ,  A61P35/00 ,  A61P37/00 ,  B82Y5/00 ,  C12N15/102 ,  C12N15/111 ,  C12N15/87 ,  C12N9/22 ,  C12N2310/14 ,  C12N2310/20 ,  C12N2310/3517 ,  C12N2320/32

摘要： Provided are methods and compositions for delivering a nucleic acid, protein, and/or ribonucleoprotein payload to a cell. Also provided are delivery molecules that include a peptide targeting ligand conjugated to a protein or nucleic acid payload (e.g., an siRNA molecule), or conjugated to a charged polymer polypeptide domain (e.g., poly-arginine such as 9R or a poly-histidine such as 6H, and the like). The targeting ligand provides for (i) targeted binding to a cell surface protein, and (ii) engagement of a long endosomal recycling pathway. As such, when the targeting ligand engages the intended cell surface protein, the delivery molecule enters the cell (e.g., via endocytosis) but is preferentially directed away from the lysosomal degradation pathway.

公开(公告)号：US20240344051A1

公开(公告)日：2024-10-17

申请号：US18754121

申请日：2024-06-25

申请人： The Regents of the University of California

发明人： Kathleen Collins ,  Heather E. Upton

IPC分类号： C12N15/10 ,  C07K14/435 ,  C12N9/12

CPC分类号： C12N15/10 ,  C07K14/43563 ,  C12N9/1276 ,  C12N2310/344 ,  C12N2310/345 ,  C12N2310/3517

摘要： The invention provides compositions and methods for nucleic acid synthesis, including ordered and continuous complementary DNA (cDNA) synthesis across non-continuous templates using a modified eukaryotic non-long terminal repeat reverse transcriptase (non-LTR RT) protein.

公开(公告)号：US20240344050A1

公开(公告)日：2024-10-17

申请号：US18754118

申请日：2024-06-25

申请人： The Regents of the University of California

发明人： Kathleen Collins ,  Heather E. Upton

IPC分类号： C12N15/10 ,  C07K14/435 ,  C12N9/12

CPC分类号： C12N15/10 ,  C07K14/43563 ,  C12N9/1276 ,  C12N2310/344 ,  C12N2310/345 ,  C12N2310/3517

摘要： The invention provides compositions and methods for nucleic acid synthesis, including ordered and continuous complementary DNA (cDNA) synthesis across non-continuous templates using a modified eukaryotic non-long terminal repeat reverse transcriptase (non-LTR RT) protein.

公开(公告)号：US20240287502A1

公开(公告)日：2024-08-29

申请号：US18397280

申请日：2023-12-27

申请人： Thermo Fisher Scientific Baltics UAB ,  Life Technologies Corporation

发明人： Arvydas Lubys ,  Inga Cikotiene ,  Zana Kapustina ,  Arturas Berezniakovas ,  Justina Medziune ,  Simona Zeimyte ,  Mark Andersen ,  Michael Allen ,  Sihong Chen ,  Arseny Smirnov ,  Bryce Alves ,  Paul Rack

IPC分类号： C12N15/10 ,  C12N15/115 ,  C12Q1/6806

CPC分类号： C12N15/1065 ,  C12N15/115 ,  C12Q1/6806 ,  C12N2310/3517

摘要： The present disclosure describes oligonucleotide-tethered nucleotides, methods of making them, and methods of using them. The oligonucleotide-tethered nucleotides comprise, in some embodiments, a nucleotide linked to an oligonucleotide of from about 3 to about 100 nucleotides in length. These oligonucleotide-tethered nucleotides can be used to label a plurality of different types of nucleic acids in a plurality of different situations with a known oligonucleotide, which can serve as a barcode in some embodiments. The resulting oligonucleotide-labeled nucleic acids oligonucleotides can be used in a variety of nucleic acid sequencing methods.

公开(公告)号：US12071652B2

公开(公告)日：2024-08-27

申请号：US17323695

申请日：2021-05-18

申请人： Purdue Research Foundation

发明人： Casey J. Krusemark ,  Kyle Denton ,  Dongwook Kim ,  Rachael Jetson

IPC分类号： C12Q1/6823 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/686 ,  G01N33/68

CPC分类号： C12Q1/6823 ,  C07H21/04 ,  C12N15/1065 ,  C12N15/1075 ,  C12Q1/686 ,  G01N33/68 ,  C12N2310/3517 ,  C12N2320/10 ,  C12N15/1075 ,  C12Q2521/537 ,  C12Q2563/179 ,  C12Q1/6823 ,  C12Q2521/537 ,  C12Q2563/179

摘要： Systems, kits, and methods for detecting and quantifying proteomic activity using DNA-encoded probes are provided, where the proteomic activity may be enzymatic activity or ligand binding affinity. Such systems and methods encode quantitative proteomic activity information into DNA sequence populations and utilize DNA-linked substrates or ligands as activity probes. The systems, kits, and methods that are directed to detecting ligand affinity further include crosslinking steps to ensure the integrity of the DNA-linked ligands during purification and washing. Signal detection involves the chemical manipulation of a probe population downstream of sample exposure and application of purifying, selective pressure for desired products. Selection-induced changes in DNA abundance between the initial pool and the purified pool indicate sample activity.

公开(公告)号：US11898195B2

公开(公告)日：2024-02-13

申请号：US17466474

申请日：2021-09-03

申请人： The University of Chicago ,  National Centre for Biological Sciences

发明人： Yamuna Krishnan ,  Saheli Halder

IPC分类号： C12N15/11 ,  C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6876 ,  C12Q1/6809 ,  C12N15/113 ,  G01N33/84 ,  C12Q1/00 ,  C12Q1/02

CPC分类号： C12Q1/6809 ,  C12N15/111 ,  C12N15/113 ,  C12Q1/005 ,  C12Q1/025 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6876 ,  G01N33/84 ,  C12N2310/14 ,  C12N2310/3517 ,  C12Q1/6816 ,  C12Q2525/205 ,  C12Q2527/119 ,  C12Q1/6825 ,  C12Q2525/205 ,  C12Q2527/119 ,  C12Q2565/1015

摘要： Described herein are nucleic acid molecules and complexes useful as i-switch pH reporters that have increased sensitivities as a pH reporter and have alternate pH reporting capacity ranges. Aspects of the disclosure relate to a method for determining pH comprising providing a nucleic acid complex comprising: a first single-stranded nucleic acid molecule comprising the sequence CnXCnYCnZCn (SEQ ID NO. 6) wherein C is cytosine; X, Y and Z are each one or more of adenine, thymine, guanine, or combinations thereof; and n is greater than or equal to 2; and wherein at least 2 cytosine residues of the first single-stranded nucleic acid molecule are modified; and a second single-stranded nucleic acid molecule that is partially or fully complementary to the first single-stranded molecule, wherein a first label is conjugated to the first single-stranded nucleic acid molecule or the second single-stranded nucleic acid molecule; and wherein the first label is capable of producing a signal, wherein the intensity of the signal varies as a function of the conformation of the nucleic acid complex; and measuring the intensity of the signal and determining the pH from the measured signal.

公开(公告)号：US20240044865A1

公开(公告)日：2024-02-08

申请号：US18357504

申请日：2023-07-24

申请人： Oxford Nanopore Technologies PLC

发明人： Andrew John Heron ,  James Edward Graham ,  Melania Slawa Strycharska

IPC分类号： G01N33/487 ,  C12N15/11 ,  G01N33/68

CPC分类号： G01N33/48721 ,  C12N15/11 ,  G01N33/6818 ,  C12N2310/3517 ,  G01N2458/00

摘要： Provided herein are methods of characterising a target polypeptide as it moves with respect to a nanopore. Also provided are related kits, systems and apparatuses for carrying out such methods.

公开(公告)号：US20230416733A1

公开(公告)日：2023-12-28

申请号：US18332464

申请日：2023-06-09

申请人： Agilent Technologies, Inc.

发明人： Daniel E. RYAN ,  Douglas J. DELLINGER ,  Jeffrey R. SAMPSON ,  Robert KAISER ,  Joel MYERSON

IPC分类号： C12N15/11 ,  C12N9/22 ,  C07H21/02 ,  C12N15/113 ,  C12N15/90 ,  C12Q1/6876

CPC分类号： C12N15/111 ,  C12N9/22 ,  C07H21/02 ,  C12N15/113 ,  C12N15/907 ,  C12Q1/6876 ,  C12N2310/10 ,  C12N2310/312 ,  C12N2310/323 ,  C12N2310/333 ,  C12N2310/346 ,  C12N2310/3517 ,  C12N2320/51 ,  C12N2320/53 ,  C12N2330/31 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/3231 ,  C12N2310/335 ,  C12N2310/531 ,  C12N2310/321 ,  C12N2310/322

摘要： The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.

公开(公告)号：US20230383289A1

公开(公告)日：2023-11-30

申请号：US18326689

申请日：2023-05-31

申请人： The Broad Institute, Inc. ,  President and Fellows of Harvard College

发明人： David R. Liu ,  Andrew Vito Anzalone ,  James William Nelson

IPC分类号： C12N15/11 ,  C12N9/12 ,  C12N9/22 ,  C12N15/90 ,  C07K14/00 ,  C12N15/62

CPC分类号： C12N15/11 ,  C12N9/1276 ,  C12N9/22 ,  C12N15/907 ,  C12Y207/07049 ,  C07K14/001 ,  C12N15/62 ,  C12N15/902 ,  C12N2310/20 ,  C07K2319/00 ,  C12N2310/3519 ,  C12N2310/3517 ,  C12N2800/80

摘要： Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.

公开(公告)号：US20180320183A1

公开(公告)日：2018-11-08

申请号：US15773271

申请日：2016-11-16

申请人： ANIMA BIOTECH INC. ,  THOMAS JEFFERSON UNIVERSITY

发明人： Iris ALROY ,  Ya-Ming HOU ,  Howard GAMPER

IPC分类号： C12N15/115

CPC分类号： C12N15/115 ,  C12N15/62 ,  C12N15/70 ,  C12N2310/16 ,  C12N2310/3517 ,  C12N2310/3519 ,  C12N2320/11

摘要： The present invention provides functional aptamer-comprising tRNA molecules, useful in the study of tRNA and ribosomal activity.