公开(公告)号：US20040058436A1

公开(公告)日：2004-03-25

申请号：US10455136

申请日：2003-06-05

Applicant: East China University of Science and Technology

Inventor： Yuanxing Zhang ,  Xiangming Sun ,  Weiming Fan ,  Jian Lu

IPC: C12M001/02

CPC classification number: C12M27/02 ,  C12M33/14 ,  C12M47/02

Abstract: A cell-detaching reactor is provided to prepare single cell suspension for inoculation of anchorage-dependent cells between the scaled-up bioreactors, which is especially useful in a commercial-scale process. The cell-detaching reactor of the invention comprises a trypsinizing zone and a separating zone, which are separated by a screen with mesh size between the diameters of the cells and the carriers.

Abstract translation: 提供细胞分离反应器以制备用于在放大的生物反应器之间接种锚定依赖细胞的单细胞悬浮液，其在商业规模过程中特别有用。 本发明的细胞分离反应器包括一个胰蛋白酶消化区和一个分离区，它们被网孔直径与载体直径之间的筛网隔开。