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    Endospore detection using hydrophobic collection material
    2.
    发明授权
    Endospore detection using hydrophobic collection material 有权

    公开(公告)号：US10316347B2

    公开(公告)日：2019-06-11

    申请号：US14315606

    申请日：2014-06-26

    Applicant: Ecolab USA Inc.

    Inventor： Anthony Wayne Erickson ,  Elaine Patricia Black ,  Nathan Richard Ortmann ,  Rodney H. Banks

    IPC: C12Q1/06 ,  C12Q1/24

    Abstract: In situ optical analysis of bacterial endospores can be inhibited when the endospores are present within an optically active carrier medium. To help isolate the endospores from the carrier medium, in some examples, the carrier medium is passed through a hydrophobic material that captures the endospores via hydrophobic attraction. Subsequently, a germination fluid and lanthanide source, such as terbium, can be added to the bacterial endospores captured on the hydrophobic material to form a lanthanide-dipicolinic acid complex in the germination fluid. The germination fluid can then be optically analyzed by measuring the fluorometric response of the lanthanide-dipicolinic acid complex to determine a concentration of the bacterial endospores in the carrier medium.

    ENDOSPORE DETECTION USING HYDROPHOBIC COLLECTION MATERIAL
    3.
    发明申请
    ENDOSPORE DETECTION USING HYDROPHOBIC COLLECTION MATERIAL 审中-公开
    使用疏水性收集材料进行内镜检测

    公开(公告)号：US20150376675A1

    公开(公告)日：2015-12-31

    申请号：US14315606

    申请日：2014-06-26

    Applicant: Ecolab USA Inc.

    Inventor： Anthony Wayne Erickson ,  Elaine Patricia Black ,  Nathan Richard Ortmann ,  Rodney H. Banks

    IPC: C12Q1/06

    CPC classification number: C12Q1/06 ,  C12Q1/04 ,  C12Q1/24

    Abstract: In situ optical analysis of bacterial endospores can be inhibited when the endospores are present within an optically active carrier medium. To help isolate the endospores from the carrier medium, in some examples, the carrier medium is passed through a hydrophobic material that captures the endospores via hydrophobic attraction. Subsequently, a germination fluid and lanthanide source, such as terbium, can be added to the bacterial endospores captured on the hydrophobic material to form a lanthanide-dipicolinic acid complex in the germination fluid. The germination fluid can then be optically analyzed by measuring the fluorometric response of the lanthanide-dipicolinic acid complex to determine a concentration of the bacterial endospores in the carrier medium.

    Abstract translation: 当内生孢子存在于光学活性载体介质中时，可以抑制细菌内生孢子的原位光学分析。 为了帮助从载体介质中分离内生孢子，在一些实例中，载体介质通过疏水材料，其通过疏水吸引捕获内生孢子。 随后，可将萌发液和镧系元素源（例如铽）加入捕获在疏水材料上的细菌内生孢子中，以在萌发液中形成镧系元素 - 二吡啶甲酸络合物。 然后可以通过测量镧系元素 - 二吡啶甲酸络合物的荧光响应来测定发酵液中载体介质中的细菌内生孢子的浓度来进行光学分析。

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