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    Recombinant DNA constructs and methods for controlling gene expression
    2.
    发明授权
    Recombinant DNA constructs and methods for controlling gene expression 有权
    重组DNA构建体和控制基因表达的方法

    公开(公告)号：US08217227B2

    公开(公告)日：2012-07-10

    申请号：US11545072

    申请日：2006-10-10

    申请人： Edwards Allen ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Thomas Malvar ,  James K. Roberts

    发明人： Edwards Allen ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Thomas Malvar ,  James K. Roberts

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N15/87 ,  C07H21/04

    CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

    摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

    摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。

    Recombinant DNA constructs employing site-specific recombination
    9.
    发明授权
    Recombinant DNA constructs employing site-specific recombination 有权
    使用位点特异性重组的重组DNA构建体

    公开(公告)号：US08816153B2

    公开(公告)日：2014-08-26

    申请号：US12869806

    申请日：2010-08-27

    申请人： Larry A. Gilbertson ,  Shihshieh Huang ,  Thomas Malvar

    发明人： Larry A. Gilbertson ,  Shihshieh Huang ,  Thomas Malvar

    IPC分类号： C12N15/11 ,  C12N15/82 ,  C12N5/10

    CPC分类号： C12N15/8213 ,  C12N15/63 ,  C12N15/8217 ,  C12N15/8218 ,  C12N15/8243

    摘要： Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.

    摘要翻译： 用于植物和植物细胞的重组DNA构建体具有位点特异性重组位点，其允许通过过表达或抑制内源基因来评估表型和作用模式。 在一方面，单个DNA构建体可以通过重组酶（例如Cre重组酶）对具有lox重组位点的构建体的作用在过度表达和抑制之间切换。 其他有用的重组系统包括Flp / frt系统，R / Rs系统，Dre / rox系统和GIN / gix系统。