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公开(公告)号：US09322031B2
公开(公告)日：2016-04-26
申请号：US13444802
申请日：2012-04-11
申请人： Scott E. Andersen , Yongwei Cao , Stanton B. Dotson , Michael D. Edgerton , David K. Kovalic , Linda L. Lutfiyya , Zhidong Xie , Gregory R. Heck , Thomas J. La Rosa , Jingdong Liu , Jingrui Wu , Garrett J. Lee , Thomas R. Adams , Donald E. Nelson , Timothy Conner
发明人： Scott E. Andersen , Yongwei Cao , Stanton B. Dotson , Michael D. Edgerton , David K. Kovalic , Linda L. Lutfiyya , Zhidong Xie , Gregory R. Heck , Thomas J. La Rosa , Jingdong Liu , Jingrui Wu , Garrett J. Lee , Thomas R. Adams , Donald E. Nelson , Timothy Conner
CPC分类号： C12N15/8271 , A01H1/06 , C12N15/8273
摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
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2.发明申请Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement 审中-公开核酸分子和其他与植物有关的分子及其用途，用于植物改良公开(公告)号：US20130185831A1
公开(公告)日：2013-07-18
申请号：US13507812
申请日：2012-07-31
申请人： David K. Kovalic , Scott E. Andersen , Joseph R. Byrum , Timothy W. Conner , James D. Masucci , Yihua Zhou
发明人： David K. Kovalic , Scott E. Andersen , Joseph R. Byrum , Timothy W. Conner , James D. Masucci , Yihua Zhou
IPC分类号： C07K14/415 , A01G1/00
CPC分类号： C12N15/8261 , A01G22/00 , C07K14/415 , C12N15/8243 , C12N15/8245 , C12N15/8247 , C12N15/8251 , C12N15/8255 , C12N15/8269 , C12N15/8273 , C12N15/8274 , C12N15/8279 , C12N15/902
摘要： Recombinant polynucleotides and recombinant polypeptides useful for improvement of plants are provided. The disclosed recombinant polynucleotides and recombinant polypeptides find use in production of transgenic plants to produce plants having improved properties.
摘要翻译： 提供了可用于改良植物的重组多核苷酸和重组多肽。 所公开的重组多核苷酸和重组多肽可用于生产转基因植物以产生具有改进性质的植物。
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3.发明授权Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement 有权与植物相关的核酸分子和其他分子及其用于植物改良的用途公开(公告)号：US08106174B2
公开(公告)日：2012-01-31
申请号：US11978197
申请日：2007-10-29
申请人： David K. Kovalic , Yihua Zhou , Yongwei Cao , Scott E. Andersen , Michael D. Edgerton , Jingdong Liu
发明人： David K. Kovalic , Yihua Zhou , Yongwei Cao , Scott E. Andersen , Michael D. Edgerton , Jingdong Liu
CPC分类号： C12N15/8261 , C07H21/04 , C07K14/415 , C12N15/82 , C12N15/8216 , C12N15/8218 , C12N15/8247 , C12N15/8251 , C12N15/8273 , C12N15/8274 , C12N15/8275
摘要： Recombinant polynucleotides and recombinant polypeptides useful for improvement of plants are provided. The disclosed recombinant polynucleotides and recombinant polypeptides find use in production of transgenic plants to produce plants having improved properties.
摘要翻译： 提供了可用于改良植物的重组多核苷酸和重组多肽。 所公开的重组多核苷酸和重组多肽可用于生产转基因植物以产生具有改进性质的植物。
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4.发明申请Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement 审中-公开与植物相关的核酸分子和其他分子及其用于植物改良的用途公开(公告)号：US20110277178A1
公开(公告)日：2011-11-10
申请号：US11978677
申请日：2007-10-30
申请人： Jingdong Liu , Yihua Zhou , David K. Kovalic , Steven E. Screen , Jack E. Tabaska , Yongwei Cao , Scott E. Andersen , Michael D. Edgerton , Karen L. Fincher , Kim Hammond-Kosack , Thomas J. La Rosa , James D. Masucci , Martin Urban , Jinhua Xiao , Todd E. Ziegler
发明人： Jingdong Liu , Yihua Zhou , David K. Kovalic , Steven E. Screen , Jack E. Tabaska , Yongwei Cao , Scott E. Andersen , Michael D. Edgerton , Karen L. Fincher , Kim Hammond-Kosack , Thomas J. La Rosa , James D. Masucci , Martin Urban , Jinhua Xiao , Todd E. Ziegler
CPC分类号： C07K14/415
摘要： Polynucleotides useful for improvement of plants are provided. In particular, polynucleotide sequences are provided from plant sources. Polypeptides encoded by the polynucleotide sequences are also provided. The disclosed polynucleotides and polypeptides find use in production of transgenic plants to produce plants having improved properties.
摘要翻译： 提供了可用于改良植物的多核苷酸。 特别地，从植物来源提供多核苷酸序列。 还提供了由多核苷酸序列编码的多肽。 所公开的多核苷酸和多肽可用于生产转基因植物以产生具有改进性质的植物。
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5.发明申请公开(公告)号：US20100223694A1
公开(公告)日：2010-09-02
申请号：US12726673
申请日：2010-03-18
申请人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
发明人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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公开(公告)号：US20090307803A1
公开(公告)日：2009-12-10
申请号：US11547764
申请日：2005-04-08
申请人： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R.I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
发明人： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R.I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
CPC分类号： C12N15/8286 , A01N63/02 , C12N15/8275 , C12N15/8279 , C12N15/8285 , Y02A40/162 , Y02A40/164
摘要： The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
摘要翻译： 本发明涉及通过抑制无脊椎害虫中的一种或多种生物学功能来控制害虫侵袭。 本发明公开了通过将一种或多种不同的重组双链RNA分子加入害虫来控制害虫侵染的方法和组合物，以通过抑制基因表达来实现害虫侵染的减少。 本发明还涉及用于制备表达双链RNA分子的转基因植物以及用于保护植物免受虫害侵袭的转基因杀虫剂的特定组合的方法。
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7.发明申请METHODS FOR GENETIC CONTROL OF PLANT PEST INFESTATION AND COMPOSITIONS THEREOF 有权植物病虫害感染的遗传控制方法及其组合物公开(公告)号：US20090188005A1
公开(公告)日：2009-07-23
申请号：US12101830
申请日：2008-04-11
申请人： Andrey A. Boukharov , Zijin Du , Liang Guo , David K. Kovalic , Maolong Lu , James P. McCarter , Nancy M. Miller , Mark Vaudin , Deryck Jeremy Williams , Wei Wu
发明人： Andrey A. Boukharov , Zijin Du , Liang Guo , David K. Kovalic , Maolong Lu , James P. McCarter , Nancy M. Miller , Mark Vaudin , Deryck Jeremy Williams , Wei Wu
CPC分类号： C12N15/8285 , C07K14/4354 , C12N15/8241 , C12N15/8245 , C12N15/8247 , C12N15/8274 , C12N15/8279 , Y02A40/164
摘要： The present invention is directed to controlling plant pest infestation, and particularly plant nematode infestation, by inhibiting one or more biological functions in the plant pest. The invention discloses methods and compositions for use in controlling plant pest infestation by providing one or more different recombinant double stranded RNA molecules in the diet of the pest in order to achieve a reduction in pest infestation through suppression of pest gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, to methods for detecting cells comprising the disclosed sequences, and to methods for detecting the disclosed sequences in biological samples.
摘要翻译： 本发明涉及通过抑制植物有害生物中的一种或多种生物功能来控制植物害虫侵染，特别是植物线虫侵染。 本发明公开了通过在害虫的饮食中提供一种或多种不同的重组双链RNA分子来控制植物害虫侵袭的方法和组合物，以通过抑制害虫基因表达来实现害虫侵染的减少。 本发明还涉及用于制备表达双链RNA分子的转基因植物的方法，用于检测包含所公开序列的细胞的方法，以及用于检测生物样品中所公开序列的方法。
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8.发明申请Nucleic acid molecules and other molecules associated with transcription in plants and uses thereof for plant improvement 审中-公开核酸分子和与植物转录相关的其他分子及其用于植物改良的用途公开(公告)号：US20080229439A1
公开(公告)日：2008-09-18
申请号：US11980417
申请日：2007-10-31
申请人： Thomas J. La Rosa , Linda Lutfiyya , Yihua Zhou , David K. Kovalic , Andrey A. Boukharov , Ping Li , Wei Wu , Timothy W. Conner , Jingdong Liu
发明人： Thomas J. La Rosa , Linda Lutfiyya , Yihua Zhou , David K. Kovalic , Andrey A. Boukharov , Ping Li , Wei Wu , Timothy W. Conner , Jingdong Liu
CPC分类号： C12N15/8218 , C07K14/415 , C12N15/8201 , C12N15/8222 , C12N15/8242 , C12N15/8261 , C12N15/827 , C12N15/8273 , Y02A40/146
摘要： Polynucleotides useful for improvement of plants are provided. In particular, polynucleotide sequences are provided from plant sources. Polypeptides encoded by the polynucleotide sequences are also provided. The disclosed polynucleotides and polypeptides find use in production of transgenic plants to produce plants having improved properties.
摘要翻译： 提供了可用于改良植物的多核苷酸。 特别地，从植物来源提供多核苷酸序列。 还提供了由多核苷酸序列编码的多肽。 所公开的多核苷酸和多肽可用于生产转基因植物以产生具有改进性质的植物。
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公开(公告)号：US08124844B2
公开(公告)日：2012-02-28
申请号：US11980366
申请日：2007-10-31
申请人： Thomas J. La Rosa , David K. Kovalic , Yihua Zhou , Yongwei Cao , Mark S. Abad , Scott E. Andersen , Robert E. Buehler , Joseph R. Byrum , Nordine Cheikh , Timothy W. Conner , Brian E. Coombs , Stanton B. Dotson , Patrice Dubois , Michael D. Edgerton , Dane K. Fisher , Kristine J. Hardeman , Gregory J. Heck , Gregory J. Hinkle , Raghunath V. Lalgudi , Jingdong Liu , Linda L. Lutfiyya , Debbie A. Mahadeo , James D. Masucci , James D. McIninch , Philip W. Miller , Donald E. Nelson , Ming Peng , Thomas G. Ruff , Hridayabhiranjan Shukla , Joshua Stein , Michael D. Thompson , Kunsheng Wu
发明人： Thomas J. La Rosa , David K. Kovalic , Yihua Zhou , Yongwei Cao , Mark S. Abad , Scott E. Andersen , Robert E. Buehler , Joseph R. Byrum , Nordine Cheikh , Timothy W. Conner , Brian E. Coombs , Stanton B. Dotson , Patrice Dubois , Michael D. Edgerton , Dane K. Fisher , Kristine J. Hardeman , Gregory J. Heck , Gregory J. Hinkle , Raghunath V. Lalgudi , Jingdong Liu , Linda L. Lutfiyya , Debbie A. Mahadeo , James D. Masucci , James D. McIninch , Philip W. Miller , Donald E. Nelson , Ming Peng , Thomas G. Ruff , Hridayabhiranjan Shukla , Joshua Stein , Michael D. Thompson , Kunsheng Wu
IPC分类号： C12N15/00 , A01H5/00 , A01H1/00 , C07H21/04 , C07K14/415
摘要： Polynucleotides useful for improvement of plants are provided. In particular, polynucleotide sequences are provided from plant sources. Polypeptides encoded by the polynucleotide sequences are also provided. The disclosed polynucleotides and polypeptides find use in production of transgenic plants to produce plants having improved properties.
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10.发明申请ISOLATED NOVEL NUCLEIC ACID AND PROTEIN MOLECULES FROM CORN AND METHODS OF USING THOSE MOLECULES TO GENERATE TRANSGENE PLANTS WITH ENHANCED AGRONOMIC TRAITS 审中-公开从CORN分离的新型核酸和蛋白质分子以及使用这些分子生产具有增强的农业条件的转基因植物的方法公开(公告)号：US20120017292A1
公开(公告)日：2012-01-19
申请号：US13138180
申请日：2010-01-13
申请人： David K. Kovalic , Jack E. Tabaska , Bo-Xing Qiu , Wei Wu
发明人： David K. Kovalic , Jack E. Tabaska , Bo-Xing Qiu , Wei Wu
CPC分类号： C12N15/8275 , A01H1/02 , A01H1/04 , A01H5/10 , C07K14/415 , C12N15/113 , C12N15/8209 , C12N15/8218 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8274 , C12N2310/11 , Y02A40/146
摘要： This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.
摘要翻译： 本公开提供了纯化的核酸和多肽。 还提供了转基因植物，种子和含有用于表达可用于赋予转基因作物植物增强的农学性状的蛋白质的DNA的植物细胞，以及制造这种植物的方法以及制造包括种子和杂交种子在内的农产品的方法 从这样的植物。
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