公开(公告)号：US11915444B2

公开(公告)日：2024-02-27

申请号：US17854042

申请日：2022-06-30

Applicant: Element Biosciences, Inc.

Inventor： Chunhong Zhou ,  Semyon Kruglyak ,  Francisco Garcia ,  Minghao Guo ,  Haosen Wang ,  Ryan Kelly

IPC: G06T7/66 ,  G06T7/30 ,  G06V10/75 ,  G06V20/69 ,  G06F18/232 ,  G06V10/762

CPC classification number: G06T7/66 ,  G06F18/232 ,  G06T7/30 ,  G06V10/751 ,  G06V10/763 ,  G06V20/69 ,  G06V20/693 ,  G06V20/695 ,  G06V2201/04

Abstract: Methods and systems for image analysis are provided, and in particular for identifying a set of base-calling locations in a flow cell for DNA sequencing. These include capturing flow cell images after each sequencing step performed on the flow cell, and identifying candidate cluster centers in at least one of the flow cell images. Intensities are determined for each candidate cluster center in a set of flow cell images. Purities are determined for each candidate cluster center based on the intensities. Each candidate cluster center with a purity greater than the purity of the surrounding candidate cluster centers within a distance threshold is added to a template set of base-calling locations.

公开(公告)号：US11200446B1

公开(公告)日：2021-12-14

申请号：US17219556

申请日：2021-03-31

Applicant: Element Biosciences, Inc.

Inventor： Chunhong Zhou ,  Semyon Kruglyak ,  Francisco Garcia ,  Minghao Guo ,  Haosen Wang ,  Ryan Kelly

IPC: G06K9/62 ,  G06K9/00 ,  G06T7/30

Abstract: Methods and systems for image analysis are provided, and in particular for identifying a set of base-calling locations in a flow cell for DNA sequencing. These include capturing flow cell images after each sequencing step performed on the flow cell, and identifying candidate cluster centers in at least one of the flow cell images. Intensities are determined for each candidate cluster center in a set of flow cell images. Purities are determined for each candidate cluster center based on the intensities. Each candidate cluster center with a purity greater than the purity of the surrounding candidate cluster centers within a distance threshold is added to a template set of base-calling locations.