Determining interactions between cells based on force spectroscopy

    公开(公告)号:US12125299B2

    公开(公告)日:2024-10-22

    申请号:US17755610

    申请日:2020-11-04

    摘要: Methods and systems for determining interaction between cells are described wherein the method includes determining or receiving a sequence of images representing manipulating first cells, in a holding space, the holding space including a functionalized wall comprising second cells, the manipulating including settling of the first cells onto the functionalized wall and applying a force on the settled first cells; detecting groups of pixels representing first cells in first images representing the settling of the first cells onto the functionalized wall; tracking locations of detected first cells in the first images; and, determining settling events, a settling event being determined if a cell in a first image is not distinguishable from background of the first image, the location in the image at which a cell settling event is detected defining a cell settling location; detecting groups of pixels representing cells in second images captured during the application of the force and tracking locations of detected cells, wherein tracked locations of a detected cell in the second images form a tracking path, the first location of the tracking path defining a pop-up event, the location in a second image at which a pop-up event is detected defining a pop-up location; and, determining detachment events based on the settling locations and based on the pop-up locations, a detachment event defining a first cell being detached from a second cell due to application of the force on the first cell, and determining information about the interaction between first and second cells based on the force applied to the first cells.

    Distributed retrieval method, apparatus, and system, computer device, and storage medium

    公开(公告)号:US12125258B2

    公开(公告)日:2024-10-22

    申请号:US17665404

    申请日:2022-02-04

    IPC分类号: G06V10/75 G06T1/60 G06V10/74

    摘要: Embodiments of this application disclose a distributed retrieval method performed by a distributed retrieval system including a retrieval node and an access node. The retrieval node receives a retrieval request forwarded by an access node, at least two video memory blocks are searched for matching features matching a to-be-detected feature of an image, each video memory block being a unique physical storage unit with a fixed length, and then returns retrieval results to the access node, the retrieval results being used for indicating the features matching the to-be-detected feature. Because cache spaces are allocated between the at least two video memory blocks using a blockchain structure, the features can be continuously stored in the video memory blocks, thereby overcoming the problem of data discretization brought about by a storage hole caused by deletion in the related art.