公开(公告)号：US20240011022A1

公开(公告)日：2024-01-11

申请号：US18347440

申请日：2023-07-05

Applicant: Element Biosciences, Inc.

Inventor： Junhua ZHAO ,  Xiaodong QI ,  Shawn LEVY

IPC: C12N15/10

CPC classification number: C12N15/1065

Abstract: The present disclosure provides compositions comprising nucleic acid double-stranded splint adaptors, including kits, and methods that employ the double-stranded splint adaptors, e.g., PCR-free workflows. The double-stranded splint adaptors (200) can be used in a one-pot, multi-enzyme reaction to introduce one or more new adaptor sequences into a library molecule. The double-stranded splint adaptor (200) comprises a first splint strand (long splint strand (300)) and a second splint strand (short splint strand (400)), where the first and second splint strands are hybridized together to form the double-stranded splint adaptor (200) having a double-stranded region and two flanking single-stranded regions. The second splint strand (400) carries the new adaptor sequence(s) to be introduced, such as for example a universal binding sequence and/or an index sequence.

公开(公告)号：US20240052398A1

公开(公告)日：2024-02-15

申请号：US18450200

申请日：2023-08-15

Applicant: Element Biosciences, Inc.

Inventor： Michael PREVITE ,  Shawn LEVY

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6844

CPC classification number: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6844

Abstract: The present disclosure provides compositions, apparatuses and methods for capturing on a support nucleic acids from cellular samples, preparing library molecules on the support, amplifying the library molecules on the support to generate nucleic acid template molecules, and analyzing the immobilized nucleic acid template molecules including detecting and/or sequencing the immobilized nucleic acid template molecules. The immobilized nucleic acid template molecules correspond to the nucleic acids from the cellular samples. The immobilized nucleic acid template molecules are spatially located on the support at positions that correspond to the spatial location of the nucleic acids from the cellular sample.