公开(公告)号：US20210223178A1

公开(公告)日：2021-07-22

申请号：US17151029

申请日：2021-01-15

Applicant: Element Biosciences, Inc.

Inventor： Minghao Guo ,  Michael Previte ,  Steven Xiangling Chen ,  Chunhong Zhou

IPC: G01N21/64 ,  C12Q1/6869

Abstract: Optical systems for DNA sequencing and other assays are described. Microscope designs may include a light source configured to emit an excitation beam and an objective lens disposed to receive the excitation beam, direct the excitation beam to a specimen, and receive emission light emitted by the specimen in response to the excitation beam. A plurality of detection channels includes optics configured to receive at least a portion of the emission light. A first dichroic filter can be disposed to reflect the excitation beam into the objective lens and to transmit the emission light, and a second dichroic filter can be disposed to receive the transmitted emission light, transmit a first portion of the transmitted emission light to a first channel of the plurality of channels, and reflect a second portion of the transmitted emission light to a second channel of the plurality of channels. Imaging or detection performance may further be improved by a reduced angle of incidence between the emission light and some or all of the dichroic filters, and/or by linearly polarizing the excitation beam such that the excitation beam is s-polarized with respect to the first dichroic filter.

公开(公告)号：US20210223531A1

公开(公告)日：2021-07-22

申请号：US17151020

申请日：2021-01-15

Applicant: Element Biosciences, Inc.

Inventor： Minghao Guo ,  Michael Previte ,  Steven Xiangling Chen ,  Chunhong Zhou

IPC: G02B21/16 ,  B01L3/00 ,  G01N21/64 ,  G02B21/06 ,  G02B21/02

Abstract: Optical systems for DNA sequencing and other assays are described. Microscope designs may include a light source configured to emit an excitation beam and an objective lens disposed to receive the excitation beam, direct the excitation beam to a specimen, and receive emission light emitted by the specimen in response to the excitation beam. A plurality of detection channels includes optics configured to receive at least a portion of the emission light. A first dichroic filter can be disposed to reflect the excitation beam into the objective lens and to transmit the emission light, and a second dichroic filter can be disposed to receive the transmitted emission light, transmit a first portion of the transmitted emission light to a first channel of the plurality of channels, and reflect a second portion of the transmitted emission light to a second channel of the plurality of channels. Imaging or detection performance may further be improved by a reduced angle of incidence between the emission light and some or all of the dichroic filters, and/or by linearly polarizing the excitation beam such that the excitation beam is s-polarized with respect to the first dichroic filter.

公开(公告)号：US20210223530A1

公开(公告)日：2021-07-22

申请号：US16902188

申请日：2020-06-15

Applicant: Element Biosciences, Inc.

Inventor： Minghao Guo ,  Michael Previte ,  Steven Xiangling Chen ,  Chunhong Zhou

IPC: G02B21/16 ,  G02B21/02 ,  G02B21/36 ,  G02B21/06 ,  G01N21/64

Abstract: Multi-channel fluorescence microscopes and optical systems may include a light source configured to emit an excitation beam and an objective lens disposed to receive the excitation beam, direct the excitation beam to a specimen, and receive emission light emitted by the specimen in response to the excitation beam. A plurality of detection channels include optics configured to receive at least a portion of the emission light. A first dichroic filter can be disposed to reflect the excitation beam into the objective lens and to transmit the emission light, and a second dichroic filter can be disposed to receive the transmitted emission light, transmit a first portion of the transmitted emission light to a first channel of the plurality of channels, and reflect a second portion of the transmitted emission light to a second channel of the plurality of channels.