公开(公告)号：US10539497B2

公开(公告)日：2020-01-21

申请号：US15315698

申请日：2015-06-02

Applicant: Essen Instruments, Inc.

Inventor： Andrew W. Smolak ,  Erica Dawson Tenent ,  Kathy L. Rowlen ,  Garrett S. Wilson ,  Christopher H. Converse ,  Evan M. Toth

IPC: G01N15/14 ,  G01N21/64

Abstract: A method and a flow cytometer system in which a light path is aligned to optimally direct light from a light source to a flow cell of a flow cytometer instrument are provided. The flow cytometer may include an orientable mirror disposed in the light path between the light source and the flow cell. By changing an orientation of the mirror through a sequence of different orientations, an optimal orientation of the mirror may be determined, and the mirror may be oriented accordingly before proceeding to conduct a flow cytometry investigation.

公开(公告)号：US10520420B2

公开(公告)日：2019-12-31

申请号：US16206350

申请日：2018-11-30

Applicant: Essen Instruments, Inc.

Inventor： Andrew W. Smolak ,  Jean-Luc Fraikin ,  Erica Dawson Tenent ,  Kathy L. Rowlen

IPC: G01N15/14 ,  G01N15/10

Abstract: Methods of evaluating particle attributes in a sample fluid subjected to flow cytometry investigation in a flow cytometer instrument, methods of processing time series signal data traces output by a flow cytometer instrument, and a flow cytometer system are provided. In the methods and systems, data points comprising time series signal data traces corresponding with detection during the flow cytometry investigation of light from the sample fluid in one or more wavelength ranges indicative of the presence of one or more particle attributes in the sample fluid are batch-processed using a batch-specific signal peak threshold determined as a function of a batch-specific noise characteristic to identify signal peaks in the batch of data points indicative of the presence of the one or more particle attributes in the sample fluid.