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1.发明授权Continuous process for substrate modification using a packed bed column of immobilized enzyme 有权使用固定化酶的填充床柱进行底物修饰的连续方法公开(公告)号：US08703442B2
公开(公告)日：2014-04-22
申请号：US12902241
申请日：2010-10-12
CPC分类号： C12P7/6481 , C12P7/6436 , C12P7/6454
摘要： A process for the modification of a substrate comprising passing the substrate through a packed bed column of a specific volume of immobilized enzyme wherein the substrate enters the column at or near one end of the column (the ‘inlet end’) and the modified substrate exits at or near the opposite end of the column (the ‘outlet end’), a portion of the volume of immobilized enzyme is periodically removed at or near to the inlet end of the column, and an equivalent portion of immobilized enzyme is periodically added at or near to the outlet end of the column.
摘要翻译： 一种用于修饰底物的方法，包括使基底通过特定体积的固定化酶的填充床柱，其中基底在塔的一端或其一端进入塔（“入口端”）并且改性基底离开 在柱的相对端（“出口端”）处或其附近，固定化酶体积的一部分在塔的入口端处或其附近被周期性地去除，并且固定化酶的等同部分周期性地添加在 或靠近柱的出口端。
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公开(公告)号：US20110027828A1
公开(公告)日：2011-02-03
申请号：US12902241
申请日：2010-10-12
IPC分类号： C12P1/00
CPC分类号： C12P7/6481 , C12P7/6436 , C12P7/6454
摘要： A process for the modification of a substrate comprising passing the substrate through a packed bed column of a specific volume of immobilized enzyme wherein the substrate enters the column at or near one end of the column (the ‘inlet end’) and the modified substrate exits at or near the opposite end of the column (the ‘outlet end’), a portion of the volume of immobilized enzyme is periodically removed at or near to the inlet end of the column, and an equivalent portion of immobilized enzyme is periodically added at or near to the outlet end of the column.
摘要翻译： 一种用于修饰底物的方法，包括使基底通过特定体积的固定化酶的填充床柱，其中基底在塔的一端或其一端进入塔（“入口端”）并且改性基底离开 在柱的相对端（“出口端”）处或其附近，一部分固定化酶的体积在柱的入口端处或其附近定期去除，并且固定化酶的等同部分周期性地添加在 或靠近柱的出口端。
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