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    Oligonucleotide analogs, their preparation and use
    1.
    发明授权
    Oligonucleotide analogs, their preparation and use 失效
    寡核苷酸类似物，其制备和用途

    公开(公告)号：US6033909A

    公开(公告)日：2000-03-07

    申请号：US281203

    申请日：1994-07-27

    申请人： Eugen Uhlmann ,  Anuschirwan Peyman ,  Gerard O'Malley ,  Matthias Helsberg ,  Irvin Winkler

    发明人： Eugen Uhlmann ,  Anuschirwan Peyman ,  Gerard O'Malley ,  Matthias Helsberg ,  Irvin Winkler

    IPC分类号： C07H21/00 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C07H21/00 ,  C07B2200/11

    摘要： The invention relates to compounds of the formula I ##STR1## where R.sup.1 is H, alkyl, acyl, aryl, or a phosphate residue; R.sup.2 is H, OH, alkoxy, NH.sub.2, or halogen; B is a base customary in nucleotide chemistry; a is O or CH.sub.2 ; n is an integer from 1 to 100; W=O, S or Se; V=O, S, or NH; Y=O, S, NH, or CH.sub.2 ; Y'=O, S, NH, or alkylene; X=OH or SH; U=OH, SH, SeH, alkyl, alkoxy, aryl, aryloxy, or amine, and Z=OH, SH, SeH, an optionally substituted radical from the group comprising alkyl, aryl, heteroaryl, alkoxy, or amino, or a group which favors intracellular uptake or serves as the label of a DNA probe or attacks the target nucleic acid during hybridization, where if Z=OH, SH, CH.sub.3, or OC.sub.2 H.sub.5, at least one of the groups X, Y, Y', V, or W is not OH or O or R.sup.1 is not H; a process for their preparation and their use as inhibitors of gene expression, as probes for detecting nucleic acids and as aids in molecular biology.

    摘要翻译： 本发明涉及式Ⅰ化合物，其中R1是H，烷基，酰基，芳基或磷酸酯残基; R2是H，OH，烷氧基，NH2或卤素; B是核苷酸化学中常规的基础; a是O或CH2; n为1〜100的整数， W = O，S或Se; V = O，S或NH; Y = O，S，NH或CH 2; Y'= O，S，NH或亚烷基; X = OH或SH; U = OH，SH，SeH，烷基，烷氧基，芳基，芳氧基或胺，Z = OH，SH，SeH，任选取代的基团，其包含烷基，芳基，杂芳基，烷氧基或氨基， 其有利于细胞内吸收或用作DNA探针的标记或在杂交期间攻击靶核酸，其中如果Z = OH，SH，CH 3或OC 2 H 5，X，Y，Y'，V， 或W不是OH或O或R 1不是H; 其制备方法及其作为基因表达抑制剂的用途，作为检测核酸的探针和分子生物学的辅助工具。