公开(公告)号：US5783453A

公开(公告)日：1998-07-21

申请号：US496549

申请日：1995-06-29

申请人： Eve H. Barlow ,  Eddie Carroll, III ,  Joseph E. Connolly ,  Michael J. Lee ,  Richard A. Martinelli ,  John T. Unger

发明人： Eve H. Barlow ,  Eddie Carroll, III ,  Joseph E. Connolly ,  Michael J. Lee ,  Richard A. Martinelli ,  John T. Unger

IPC分类号： G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  C12G1/00 ,  G01N33/551 ,  G01N33/553

CPC分类号： G01N33/542 ,  G01N33/582

摘要： The assay described herein is predicated on an observation that when acridinium ester labeled tracers are bound to their corresponding binding conjugate immobilized on a metal oxide solid phase, the measurable chemiluminescent light emission of the labeled tracer bound to the solid phase is quenched as compared to the free fraction tracer that is unattached to the solid phase. According to the invention, a non-separation specific binding assay to detect or quantify the presence of an analyte is provided where the entire reaction mixture is flashed (including unreacted tracer) and modulated signal (because of the quench effect) is associated with a reference, thus determining the amount or presence of said analyte in said sample. Disadvantages inherent in heterogeneous assays employing multiple separations may be avoided using this non-separation method.

摘要翻译： 本文所述的测定是基于以下观察结果：当吖啶酯标记的示踪剂与其固定在金属氧化物固相上的相应结合缀合物结合时，与固相结合的标记示踪剂的可测量化学发光光发射与 与固相无关的游离级分示踪剂。 根据本发明，提供了检测或定量分析物存在的非分离特异性结合测定法，其中整个反应混合物闪蒸（包括未反应的示踪剂）和调制信号（由于骤冷作用）与参考文献相关联 ，从而确定所述样品中所述分析物的量或存在。 使用这种非分离方法可以避免使用多次分离的异质测定中固有的缺点。

公开(公告)号：US5672475A

公开(公告)日：1997-09-30

申请号：US375466

申请日：1995-01-20

申请人： Michael J. Lee ,  Howard H. Weetall ,  Joseph E. Connolly

发明人： Michael J. Lee ,  Howard H. Weetall ,  Joseph E. Connolly

IPC分类号： G01N21/76 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/68 ,  G01N33/53

CPC分类号： G01N33/582 ,  Y10S435/968 ,  Y10S435/973

摘要： A method is provided for detecting or quantitating each of a plurality of substances or at least one substance and an internal reference material or control material in a test sample using at least two different luminescent labelled conjugates. Each luminescent labelled conjugate being characterized in being activated to emit light under different process conditions. The invention also provides for test kits containing at least two different luminescent labelled conjugates for detecting or quantitating presence or absence of at least two substances or at least one substance an internal reference material or control material in a test sample.

摘要翻译： 提供一种用于使用至少两种不同的发光标记的缀合物来检测或定量测试样品中的多种物质或至少一种物质和内部参考物质或对照物质中的每一种的方法。 每个发光的标记共轭物的特征在于被激活以在不同的工艺条件下发光。 本发明还提供了含有至少两种不同的发光标记的缀合物的测试试剂盒，用于在测试样品中检测或定量存在或不存在至少两种物质或至少一种物质内部参考物质或对照物质。