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    Reduction of background in noncompetitive binding assays
    1.
    发明授权
    Reduction of background in noncompetitive binding assays 失效
    减少非竞争性结合测定中的背景

    公开(公告)号：US5705338A

    公开(公告)日：1998-01-06

    申请号：US434743

    申请日：1995-05-04

    申请人： Uri Piran ,  Laurie Ann Livshin ,  Richard A. Martinelli ,  William J. Riordan ,  John T. Unger

    发明人： Uri Piran ,  Laurie Ann Livshin ,  Richard A. Martinelli ,  William J. Riordan ,  John T. Unger

    IPC分类号： C12Q1/68 ,  G01N33/00 ,  G01N33/543 ,  G01N33/53 ,  G01N33/547

    CPC分类号： C12Q1/6813 ,  C12Q1/6834 ,  G01N33/54306 ,  G01N2033/008

    摘要： Novel non-competitive assay techniques have been developed which not only improve sensitivity, but also are convenient and less susceptible to interfering factors. They are compatible with existing instruments and can be run in one or more test tubes. The analyte is reacted with labeled specific binder, after which the mixture is reacted with (1) an insoluble material attached to an analyte derivative and (2) a solid phase carrying a binder. The solid phase is then separated, and the label attached to the solid phase is measured. Variations of the procedure include the use of a reversible bridge for attaching the insoluble material to the analyte mimic and the conduct of the assay in various porous media, such as paper, chromatographic and electrophoretic media, and dipsticks.

    摘要翻译： 已经开发了新的非竞争性测定技术，其不仅提高灵敏度，而且方便且不易受干扰因素的影响。 它们与现有仪器兼容，可以在一个或多个试管中运行。 分析物与标记的特异性粘合剂反应，之后混合物与（1）与分析物衍生物连接的不溶性物质和（2）携带粘合剂的固相反应。 然后分离固相，测量附着于固相的标记。 方法的变化包括使用可逆桥接器将不溶性物质连接到分析物模拟物上，以及在各种多孔介质如纸张，色谱和电泳介质以及浸渍条中进行测定。

    Collapsible Fire Pit and Propane Tank Holder
    2.
    发明申请
    Collapsible Fire Pit and Propane Tank Holder 审中-公开
    可折叠火坑和丙烷罐架

    公开(公告)号：US20120222668A1

    公开(公告)日：2012-09-06

    申请号：US13039804

    申请日：2011-03-03

    申请人： John T. Unger

    发明人： John T. Unger

    IPC分类号： F24B1/181 ,  F24B1/192

    CPC分类号： F24C3/14 ,  F24B1/181

    摘要： A fire pit designed to be stored and shipped in a flat configuration, and assembled without tools in a matter of minutes into a freestanding, durable structure capable of functioning as well or better than a non-collapsible fire pit. The fire pit comprises an essentially flat base with a unique slot and flange structure adapted to receive and support side panels. The side panels include female sides with vertical corner members on their side edges that allow flat male sides to mate securely with the female sides, and with the intersecting edges of the sides both covered and reinforced for a neat appearance and robust structure. A top includes a slot and flange structure that fits over and unifies and hides the upper edges of the sides, completing the assembly.

    摘要翻译： 一个防火坑被设计成以平坦的形式储存和运输，并且在几分钟之内就没有工具组装成独立的，耐用的结构，能够比不可折叠的火坑发挥好的作用。 火坑包括基本上平坦的底座，其具有适于容纳和支撑侧板的独特的槽和凸缘结构。 侧板包括在其侧边缘上具有垂直拐角构件的阴侧面，其允许平坦的阳侧与母体侧面牢固地配合，并且侧面的相交边缘被覆盖和加强，以便整洁的外观和坚固的结构。 顶部包括狭槽和凸缘结构，其适合并且组合和隐藏侧边的上边缘，从而完成组装。

    Conformational Epitope Initiated Signal Amplification
    3.
    发明申请
    Conformational Epitope Initiated Signal Amplification 审中-公开
    构象表位启动信号放大

    公开(公告)号：US20120040337A1

    公开(公告)日：2012-02-16

    申请号：US13263367

    申请日：2010-04-09

    申请人： John T. Unger ,  Rolf Hilfiker

    发明人： John T. Unger ,  Rolf Hilfiker

    IPC分类号： G01N21/64 ,  C12Q1/70 ,  G01N21/75

    CPC分类号： G01N33/557 ,  C12Q1/6804 ,  C12Q2565/101 ,  C12Q2525/205

    摘要： This invention relates to a method to generate a signal used to detect the presence or quantity of a biomarker in a sample. The signal generating reaction is initiated when the biomarker under assay interacts with a specific binding partner. The interaction produces a structural change in the binding partner that is recognized by additional binding partners capable of generating a signal. The reaction produces a localized cluster of signaling molecules that can be detected above background. The signaling cluster is detectable within minutes when interrogated in a chamber of specific dimensions. The presence of the signaling clusters is a qualitative indication of the presence of the analyte, while the number of signaling clusters detected is a direct quantification of the number of biomarker molecules in the sample. The reaction can be formatted to detect proteins, nucleic acids, cells or other informative biomarkers.

    摘要翻译： 本发明涉及生成用于检测样品中生物标志物的存在或数量的信号的方法。 当测定中的生物标志物与特异性结合配偶体相互作用时，起始信号产生反应。 相互作用产生由能够产生信号的另外的结合配偶体识别的结合配偶体的结构变化。 该反应产生可以在背景以上检测到的信号分子的局部簇。 当在特定尺寸的室中询问时，信号簇在几分钟内可被检测。 信号簇的存在是分析物存在的定性指示，而检测到的信号簇的数量是对样品中生物标记分子数量的直接定量。 该反应可被格式化以检测蛋白质，核酸，细胞或其他信息生物标志物。

    Immunoassay for class specific immunoglobulin antibodies
    6.
    发明授权
    Immunoassay for class specific immunoglobulin antibodies 失效
    针对类别特异性免疫球蛋白抗体的免疫测定

    公开(公告)号：US4434227A

    公开(公告)日：1984-02-28

    申请号：US346662

    申请日：1982-02-08

    申请人： John T. Unger

    发明人： John T. Unger

    IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/68 ,  G01N33/54

    CPC分类号： G01N33/6854 ,  Y10S435/962 ,  Y10S436/825

    摘要： A method for determining an immunoglobulin of the IgX class in a sample where X is either M, A, D or E. Anti-IgG is added to IgG to prevent binding of rheumatoid factor before the sample containing IgX is added to insolubilized IgG.

    摘要翻译： 用于确定X是M，A，D或E的样品中IgX类免疫球蛋白的方法。将抗IgG加入到IgG中，以防止在将含有IgX的样品加入到不溶性IgG中之前结合类风湿因子。

    Non-separation specific binding chemiluminescent assay
    7.
    发明授权
    Non-separation specific binding chemiluminescent assay 失效
    非分离特异性结合化学发光测定

    公开(公告)号：US5783453A

    公开(公告)日：1998-07-21

    申请号：US496549

    申请日：1995-06-29

    申请人： Eve H. Barlow ,  Eddie Carroll, III ,  Joseph E. Connolly ,  Michael J. Lee ,  Richard A. Martinelli ,  John T. Unger

    发明人： Eve H. Barlow ,  Eddie Carroll, III ,  Joseph E. Connolly ,  Michael J. Lee ,  Richard A. Martinelli ,  John T. Unger

    IPC分类号： G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  C12G1/00 ,  G01N33/551 ,  G01N33/553

    CPC分类号： G01N33/542 ,  G01N33/582

    摘要： The assay described herein is predicated on an observation that when acridinium ester labeled tracers are bound to their corresponding binding conjugate immobilized on a metal oxide solid phase, the measurable chemiluminescent light emission of the labeled tracer bound to the solid phase is quenched as compared to the free fraction tracer that is unattached to the solid phase. According to the invention, a non-separation specific binding assay to detect or quantify the presence of an analyte is provided where the entire reaction mixture is flashed (including unreacted tracer) and modulated signal (because of the quench effect) is associated with a reference, thus determining the amount or presence of said analyte in said sample. Disadvantages inherent in heterogeneous assays employing multiple separations may be avoided using this non-separation method.

    摘要翻译： 本文所述的测定是基于以下观察结果：当吖啶酯标记的示踪剂与其固定在金属氧化物固相上的相应结合缀合物结合时，与固相结合的标记示踪剂的可测量化学发光光发射与 与固相无关的游离级分示踪剂。 根据本发明，提供了检测或定量分析物存在的非分离特异性结合测定法，其中整个反应混合物闪蒸（包括未反应的示踪剂）和调制信号（由于骤冷作用）与参考文献相关联 ，从而确定所述样品中所述分析物的量或存在。 使用这种非分离方法可以避免使用多次分离的异质测定中固有的缺点。

    Amplification of midivariant DNA templates
    9.
    发明授权
    Amplification of midivariant DNA templates 失效

    公开(公告)号：US5959095A

    公开(公告)日：1999-09-28

    申请号：US352670

    申请日：1994-12-09

    申请人： Richard A. Martinelli ,  Jeffrey J. Donahue ,  John T. Unger

    发明人： Richard A. Martinelli ,  Jeffrey J. Donahue ,  John T. Unger

    IPC分类号： C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  C12P19/34 ,  C12Q1/70

    CPC分类号： C12Q1/682 ,  C12Q1/6867

    摘要： New methods are provided for the amplification of a midivariant DNA containing an inserted target specific nucleic acid sequence(s) to enable detection of the presence of a target nucleic acid sequence in a test sample. One method employs midivariant DNA as a template for the synthesis of RNA catalyzed by QB replicase. Two midivariant DNA/probe conjugates including a nonreplicable portion of midivariant DNA and a target specific nucleic acid sequence (probe) are described. An amplification method including the steps of hybridization and ligation of the midivariant DNA/probe conjugates followed by replication of the DNA template has enabled detection of less than 200 template molecules. In a modified amplification method one of the midivariant DNA/probe conjugates further includes a RNA polymerase promoter sequence to enable transcription of the midivariant DNA template into RNA. The sequential ligation-transcription-replication method enables the detection of a single template molecule. The detectable molecule(s) are indicative of the presence of the target nucleic acid sequences in the test sample.